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. 2015 Jan 29;23(1):10.
doi: 10.1186/s40199-015-0093-x.

Angiogenic effect of the aqueous extract of Cynodon dactylon on human umbilical vein endothelial cells and granulation tissue in rat

Hamid Soraya  1 , Milad Moloudizargari  2 , Shahin Aghajanshakeri  3 , Soheil Javaherypour  4 , Aram Mokarizadeh  5 , Sanaz Hamedeyazdan  6 , Hadi Esmaeli Gouvarchin Ghaleh  7 , Peyman Mikaili  8 , Alireza Garjani  9
Affiliations

Angiogenic effect of the aqueous extract of Cynodon dactylon on human umbilical vein endothelial cells and granulation tissue in rat

Hamid Soraya et al. Daru. .

Abstract

Background: Cynodon dactylon, a valuable medicinal plant, is widely used in Iranian folk medicine for the treatment of various cardiovascular diseases such as heart failure and atherosclerosis. Moreover, its anti-diabetic, anti-cancer and anti-microbial properties have been also reported. Concerning the critical role of angiogenesis in the incidence and progression of tumors and also its protective role in cardiovascular diseases, we investigated the effects of the aqueous extract prepared from the rhizomes of C. dactylon on vascular endothelial growth factor (VEGF) expressions in Human Umbilical Vein Endothelial Cells (HUVECs) and also on angiogenesis in carrageenan induced air-pouch model in rats.

Methods: In the air-pouch model, carrageenan was injected into an air-pouch on the back of the rats and following an IV injection of carmine red dye on day 6, granulation tissue was processed for the assessment of the dye content. Furthermore, in an in vitro study, angiogenic property of the extract was assessed through its effect on VEGF expression in HUVECs.

Results: Oral administration of 400 mg/kg/day of the extract significantly increased angiogenesis (p<0.05) and markedly decreased neutrophil (p<0.05) and total leukocyte infiltration (p<0.001) into the granulation tissues. Moreover, the extract increased the expression of total VEGF in HUVECs at a concentration of (100 μl/ml).

Conclusion: The present study showed that the aqueous extract of C. dactylon promotes angiogenesis probably through stimulating VEGF expression.

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Figures

Figure 1
Figure 1
Upper trace : Effects of the aqueous extract of C. dactylon (400 mg / kg) on angiogenesis in granulation tissue versus positive control (carrageenan ; left) in the air pouch model of angiogenesis in rats. Lower trace: The effect of oral administration of aqueous extract of C. dactylon on carmine dye content (as an index of angiogenesis) in granulation tissue in the air pouch model of angiogenesis in rats. Cyno: Cynodon dactylon, Carrageen: Carrageenan. Data represented as mean ± SEM. N = 6. *P < 0.05 and ***P < 0.001 vs control group (Carrageenan).
Figure 2
Figure 2
Effect of the aqueous extract of C. dactylon at various doses on pouch fluid volume and granulation tissue weight 6 days after carrageenan injection. Cyno: Cynodon dactylon, Carrageen: Carrageenan. Data represented as mean ± SEM. N = 6–8. *p < 0.05 and ***p < 0.001 versus the carrageenan group.
Figure 3
Figure 3
The expression change of VEGF in HUVECs by flow cytometric procedure. The expression level of VEGF in PBS treated cells set as control and the expression changes following treatment with C. dactylon extract compared to it. The increased expression of VEGF in extract treated HUVECs has been shown as compared to the PBS treated ones. Histograms are representative of three separate experiments.
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References

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