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. 2015 Mar 28;51(25):5471-4.
doi: 10.1039/c4cc09660f.

DNA-aptamer gating membranes

Thomas Schäfer  1 , Veli Cengiz Özalp
Affiliations

DNA-aptamer gating membranes

Thomas Schäfer et al. Chem Commun (Camb). .

Abstract

This report describes a membrane barrier whose permeability is modulated through the recognition of a small-molecule target, adenosine triphosphate (ATP), by a DNA-aptamer. The gating function of the DNA-aptamer in the stimulus-responsive membrane was shown to be specific, concentration dependent, and reversible.

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Figures

Fig. 1
Fig. 1. Modular architecture of the small-molecule stimuli-responsive membrane. The thickness of the support structure comprising the silane and avidin layers may be adapted depending on the size of the responsive receptor molecule (aptamer) and the pore size of the base material (AAO membrane).
Fig. 2
Fig. 2. Modulation of membrane permeability, represented by degree of pore closing, through specific recognition of ATP (green) by the ATP-aptamer modified AAO-membrane. Only a minor non-specific responsiveness was observed toward GTP (yellow); the AAO-membrane modified with a mutated ATP-aptamer exhibited a negligible response upon exposure to the ATP-target (violet).
Fig. 3
Fig. 3. Left: SEM picture of pore-structure of the AAO-membrane with a nominal pore size of 20 nm in its pristine form; right: schematic on the effect of the gradual membrane surface modifications on the effective pore-size.
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