Cytotoxic Activity of Bioactive Compound 1, 2- Benzene Dicarboxylic Acid, Mono 2- Ethylhexyl Ester Extracted from a Marine Derived Streptomyces sp. VITSJK8

Abstract

Marine Streptomyces are prolific producers of majority of bioactive secondary metabolites which are used in pharmaceutical industry as effective drugs against life threatening diseases. The cytotoxic activity of the pure compound 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester (DMEHE) from marine derived actinomycete Streptomyces sp. VITSJK8 was investigated against mouse embryonic fibroblast (NIH 3T3) and human keratinocyte (HaCaT) normal cell lines, human hepatocellular liver carcinoma (HepG 2) and human breast adenocarcinoma (MCF-7) cell lines by using MTT assay. The compound DMEHE exhibited IC 50 values of 42, 100, 250 and 500 µg/ ml against HepG2, MCF-7, HaCaT and NIH 3T3 cell lines, respectively. The effect of DMEHE on the growth of cancer cell lines was expressed as the % of viability. Cell viability was recorded as 67.7%, 78.14%, 82.23% and 96. 11% in HepG2, MCF-7, HaCaT and NIH 3T3 cells, respectively. The results of the study conclude that the bioactive compound isolated from the potential isolate Streptomyces sp. VITSJK8 exhibited cytotoxic activity against HepG2 and MCF- 7 cancer cell lines and low toxicity against normal HaCaT and NIH 3T3 cell lines.

Keywords: 1; 2- benzene dicarboxylic acid; MTT assay; Streptomyces sp. VITSJK8; cytotoxicity; mono 2- ethylhexyl ester.

Fig. 1

Phylogenetic tree of Streptomyces sp. VITSJK8 based on 16S rDNA. Score bar represents one nucleotide substitution per 100 nucleotides.

Fig. 2

3D structure of 1, 2-benzene dicarboxylic acid, mono 2-ethylhexyl ester (DMEHE) modelled in Chem3D Ultra 8.0

Fig. 3

Cytotoxic activity of different concentrations of DMEHE on various cell lines. Values are mean of three independent experiments; standard error bars are shown

Fig. 4

Morphological alteration of different cell lines. Pannel A: untreated NIH 3T3 (1), HaCaT (2), MCF-7 (3) and HepG2 (4) cell lines. Pannel B: 100 µg/ ml DMEHE treated NIH 3T3 (1), HaCaT (2), MCF-7 (3) and HepG2 (4) cell lines Arrows indicate morphological changes observed.