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. 2015 Mar;42(3):288-93.
doi: 10.1111/jcpe.12372. Epub 2015 Mar 9.

Evaluation of a topical herbal patch for soft tissue wound healing: an animal study

Liat Chaushu  1 , Miron Weinreb, Ilan Beitlitum, Ofer Moses, Carlos E Nemcovsky
Affiliations

Evaluation of a topical herbal patch for soft tissue wound healing: an animal study

Liat Chaushu et al. J Clin Periodontol. 2015 Mar.

Abstract

Aim: This study evaluated the effects of a topical herbal patch (PerioPatch®) for gingival wound healing in a rat model.

Materials and methods: A mid-crestal incision was performed on each side of the edentulous anterior maxilla in 48, 6-month-old, Wistar rats. Full-thickness flaps were raised, repositioned and sutured. Four experimental groups were established: herbal patch, placebo patch, no patch and no patch and no surgery. Patches were placed immediately after surgery and replaced every 12 h for the following 3 days. Half of the animals were killed after 5 and the remaining ones after 12 days. Tissue blocks were retrieved and processed for histological and immunohistochemical evaluation. Epithelial gap, collagen contents, amount of macrophages, cellular proliferation and vascular contents were evaluated in the central incision area. Statistical analysis consisted of two-way anova.

Results: The herbal patch group presented the smallest epithelial gap at 12 days, the highest collagen content both at 5 and 12 days, a larger number of proliferating cells at day 5 and more numerous blood vessels at day 12. Macrophage number was similar in all groups.

Conclusion: Herbal patch improved wound healing in this animal model.

Keywords: animal study; collagen; epithelium; herbal; histology; immunohistochemistry; inflammation; topical; wound healing.

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Figures

Figure 1
Figure 1
Epithelial gap (mean and SD) in microns in the various experimental groups. S=study group (PP); P, placebo patch group; NC, negative control (no patch) group. #, p = 0.07 (versus the respective study group). *, p = 0.05 (versus the respective study group).
Figure 2
Figure 2
Epithelial healing in the study group 12 days after surgery. (H&E). (a) alveolar crest, (b) gingival connective tissue,(c) epithelium and incision site (arrow). (×110).
Figure 3
Figure 3
Epithelial healing in the placebo patch group 12 days after surgery. (H&E). (a) alveolar crest, (b) gingival connective tissue, (c) epithelium and incision site (arrow). (×110).
Figure 4
Figure 4
Connective tissue collagen content (%, mean and SD) in the various experimental groups. S, study group (PP); P, Placebo patch group; NC, negative control (no patch) group. I, Intact (no surgery) group. **, p < 0.001 (versus the respective study group).
Figure 5
Figure 5
Number of connective tissue macrophages (CD68 positive cells) (/mm2, mean and SD) in the various experimental groups. S, study group (PP); P, placebo patch group; NC, negative control (no patch) group. I, Intact (no surgery) group.
Figure 6
Figure 6
Number of proliferating (Ki67 positive) cells (/mm2, mean and SD) in the various experimental groups. S, study group (PP); P, placebo patch group; NC, negative control (no patch) group. I, Intact (no surgery) group. *, p = 0.05 (versus the respective study group).
Figure 7
Figure 7
Number of blood vessels (TGII positive) (/mm2, mean and SD) in the various experimental groups. S, study group (PP); P, placebo patch group; NC, negative control (no patch) group. I, Intact (no surgery) group. **, p = 0.01 (versus the respective study group).
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