Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

Oliver Otto¹, Philipp Rosendahl¹, Alexander Mietke¹, Stefan Golfier¹, Christoph Herold¹, Daniel Klaue¹, Salvatore Girardo¹, Stefano Pagliara², Andrew Ekpenyong³, Angela Jacobi¹, Manja Wobus⁴, Nicole Töpfner⁵, Ulrich F Keyser², Jörg Mansfeld¹, Elisabeth Fischer-Friedrich⁶, Jochen Guck⁷

Affiliations

¹ Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
² Cavendish Laboratory, University of Cambridge, Cambridge, UK.
³ 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Department of Physics, Creighton University, Omaha, Nebraska, USA.
⁴ Medizinische Klinik 1, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
⁵ Klinik und Poliklinik für Kinder und Jugendmedizin, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
⁶ 1] Max Planck Institute for the Physics of Complex Systems, Dresden, Germany. [2] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
⁷ 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Cavendish Laboratory, University of Cambridge, Cambridge, UK.

PMID: 25643151
DOI: 10.1038/nmeth.3281

Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

Oliver Otto et al. Nat Methods. 2015 Mar.

. 2015 Mar;12(3):199-202, 4 p following 202.

doi: 10.1038/nmeth.3281. Epub 2015 Feb 2.

Authors

Affiliations

¹ Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
² Cavendish Laboratory, University of Cambridge, Cambridge, UK.
³ 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Department of Physics, Creighton University, Omaha, Nebraska, USA.
⁴ Medizinische Klinik 1, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
⁵ Klinik und Poliklinik für Kinder und Jugendmedizin, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
⁶ 1] Max Planck Institute for the Physics of Complex Systems, Dresden, Germany. [2] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
⁷ 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Cavendish Laboratory, University of Cambridge, Cambridge, UK.

PMID: 25643151
DOI: 10.1038/nmeth.3281

Abstract

We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

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References

1. Biophys J. 2013 Nov 19;105(10):2281-8 - PubMed
1. Science. 1969 May 9;164(3880):717-9 - PubMed
1. Cancer Metastasis Rev. 2009 Jun;28(1-2):113-27 - PubMed
1. Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15696-701 - PubMed
1. Biotechnol Prog. 1990 Sep-Oct;6(5):383-90 - PubMed

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Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

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Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

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Abstract

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