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. 2016:1373:89-96.
doi: 10.1007/7651_2014_187.

Allelic Exchange

Affiliations

Allelic Exchange

McKenzie K Lehman et al. Methods Mol Biol. 2016.

Abstract

Methods used to understand the function of a gene/protein are one of the hallmarks of modern molecular genetics. The ability to genetically manipulate bacteria has become a fundamental tool in studying these organisms and while basic cloning has become a routine task in molecular biology laboratories, generating directed mutations can be a daunting task. This chapter describes the method of allelic exchange in Staphylococcus aureus using temperature-sensitive plasmids that have successfully produced a variety of chromosomal mutations, including in-frame deletions, insertion of antibiotic-resistance cassettes, and even single-nucleotide point mutations.

Keywords: Allelic exchange; Cloning; Homologous recombination; Mutation.

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