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. 2015 Jan-Feb;57(1):77-80.
doi: 10.1590/S0036-46652015000100011.

Membrane fractions from Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis

Marcelo Andreetta Corral  1 , Fabiana Martins Paula  2 , Maiara Gottardi  1 , Dirce Mary Correia Lima Meisel  2 , Pedro Paulo Chieffi  1 , Ronaldo César Borges Gryschek  1
Affiliations

Membrane fractions from Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis

Marcelo Andreetta Corral et al. Rev Inst Med Trop Sao Paulo. 2015 Jan-Feb.

Abstract
in English, Portuguese

Strongyloides venezuelensis is a parasitic nematode of rodents frequently used to obtain heterologous antigens for the immunological diagnosis of human strongyloidiasis. The aim of this study was to evaluate membrane fractions from S. venezuelensis for human strongyloidiasis immunodiagnosis. Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis. Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity. The present results suggest the possible use of membrane fractions of S. venezuelensis as an alternative antigen for human strongyloidiasis immunodiagnosis.

Strongyloides venezuelensis é um nematódeo parasita de roedores, frequentemente usado como antígeno heterólogo para o diagnóstico imunológico da estrongiloidíase humana. O objetivo deste estudo foi avaliar frações de membrana de S. venezuelensis para o imunodiagnóstico da estrongiloidíase humana. Para tanto, frações solúveis e de membrana foram obtidas em solução salina fosfato (SS e MS) e Tris-HCl (ST e MT) de larvas filarioides de S. venezuelensis. Amostras de soro de 92 indivíduos, sendo 20 com estrongiloidíase (Grupo I); 32 com outras parasitoses (Grupo II), e 40 indivíduos saudáveis (Grupo III), foram analisadas pelo teste Imunoenzimático (ELISA). As frações solúveis (SS e ST) apresentaram 90,0% e 88,9%, enquanto que as frações de membrana (MS e MT) demonstraram 95,0% e 94,4%, de sensibilidade e especificidade, respectivamente. Os resultados obtidos permitem indicar as frações de membranas como antígeno alternativo para o diagnóstico da estrongiloidíase humana.

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Figures

Fig. 1
Fig. 1. Electrophoretic profiles of antigenic fractions SS, SM and TS, TM, soluble and membrane fractions from phosphate saline and Tris-HCl, respectively; 12% SDS-PAGE stained by silver nitrate. To each antigenic fraction were used 10 µg/mL.
Fig. 2
Fig. 2. Receiver-operating characteristic curve (ROC-AUC) analysis indicating the optimum point of reactions (cut-off); LR = Likelihood ratio; AUC = Area under curve.
See this image and copyright information in PMC

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