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. 2015 Feb 5:15:10.
doi: 10.1186/s12906-015-0527-5.

Anti-nociceptive, anti-inflammatory and toxicological evaluation of Fang-Ji-Huang-Qi-Tang in rodents

Yu-Chin Lin  1 , Ching-Wen Chang  2   3 , Chi-Rei Wu  4
Affiliations

Anti-nociceptive, anti-inflammatory and toxicological evaluation of Fang-Ji-Huang-Qi-Tang in rodents

Yu-Chin Lin et al. BMC Complement Altern Med. .

Abstract

Background: Fang-Ji-Huang-Qi-Tang (abbreviated as FJHQT), composed by six medicinal herbs including Radix Stephania Tetrandra, Radix Astragali, Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae, Rhizoma Zingiberis and Fructus Ziziphi Jujubae, is a frequently Chinese prescription for treating painful and inflammatory disorders such as rheumatoid arthritis. When Radix Stephania Tetrandra was misused with Aristolochia species, acute or chronic nephropathy caused by aristolochic acid was happened. Thus, the present study was aimed to identify Radix Stephania Tetrandra and performed the pharmacological and toxicological evaluation of FJHQT extract in rodents.

Methods: Radix Stephania Tetrandra was identified by macroscopic and microscopic observation, and the content of tetrandrine in FJHQT extract was measured by high performance liquid chromatography. Then, the pharmacological activities of FJHQT extract with respect to clinical use was investigated with acetic acid-induced writhing response, formalin-induced licking response and carrageenan-induced paw edema. Finally, we evaluated the subacute toxicology of FJHQT extract after 28-day repeated oral administration in rats.

Results: Radix Stephania Tetrandra was correctly used in FJHQT extract, and the content of tetrandrine in FJHQT extract was 2.5 mg/g. FJHQT extract produced a pronounced and dose-dependent antinociceptive and anti-inflammatory effects in three above models. FJHQT extract after 28-day repeated administration did not caused any hematological, biochemical and histological change in rats.

Conclusions: We suggest that FJHQT extract is a high safety index Chinese medicine for antinociceptive and anti-inflammatory application when Radix Stephania Tetrandra was correctly used in FJHQT. Its antinociceptive and anti-inflammatory mechanism might be related to peripheral nociceptive pathway such as prostaglandins.

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Figures

Figure 1
Figure 1
Pharmacognostic photographs of Radix Tetrandria. (A) Macroscopic characteristics (B) Microscopic characteristics.
Figure 2
Figure 2
HPLC chromatograms of aqueous extract of Fang-Ji-Huang-Qi-Tang at 263 nm. (A) Standard, (B) Fang-Ji-Huang-Qi-Tang.
Figure 3
Figure 3
Effect of Fang-Ji-Huang-Qi-Tang extract (FJQHT, 25, 50 and 100 mg/kg) and indomethacin (INDO, 10 mg/kg) on (A) the acetic acid-induced writhing response, and (B) the early (0–5 min) and late phase (10–35 min) of formalin-induced licking response in mice. Each values are represented as mean ± S.E. (N = 8). *P < 0.05. ** P < 0.01. *** P < 0.001 as compared with the VEH group.
Figure 4
Figure 4
Effect of Fang-Ji-Huang-Qi-Tang extract (FJQHT, 25, 50 and 100 mg/kg) and indomethacin (INDO, 10 mg/kg) on the carrageenan-induced paw edema in rats. Each values are represented as mean ± S.E. (N = 8). * P < 0.05. ** P < 0.01,as compared with the VEH group.
Figure 5
Figure 5
Effect of Fang-Ji-Huang-Qi-Tang extract (FJQHT, 0.1, 0.5 and 1.0 g/kg) on (A) the tendency of body weight and (B) daily food intake during 28-day repeated treatment in rats. Each value are represented as mean ± S.E. (N = 8).
Figure 6
Figure 6
Histology of liver and kidney (H&E stain, 100x) in rats. (A) and (C) Section of liver and kidney from vehicle-treated rats; (B) and (D) Section of liver and kidney from Fang-Ji-Huang-Qi-Tang (FJQHT, 1.0 g/kg)-treated rats.
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