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. 2015 Apr;53(4):1395-8.
doi: 10.1128/JCM.00210-15. Epub 2015 Feb 4.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry identifies Pseudomonas aeruginosa high-risk clones

Nadège Cabrolier  1 , Marlène Sauget  2 , Xavier Bertrand  3 , Didier Hocquet  4
Affiliations

Matrix-assisted laser desorption ionization-time of flight mass spectrometry identifies Pseudomonas aeruginosa high-risk clones

Nadège Cabrolier et al. J Clin Microbiol. 2015 Apr.

Abstract

We show here that matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) accurately and quickly identified the five high-risk clones of Pseudomonas aeruginosa sequence type 111 (ST111), ST175, ST235, ST253, and ST395. The use of this screening technique by clinical microbiology laboratories may tackle the spread of high-risk clones by the quick implementation of hygiene control procedures for relevant patients.

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Figures

FIG 1
FIG 1
Distribution of the 157 STs of the isolates of Pseudomonas aeruginosa used for the typing strategy on a dendrogram built with the data of all known STs (n = 1,595). STs belonging to the training set are indicated by bold type. Isolates were genotyped by multilocus sequence typing as previous described (4). The maximum-likelihood tree was constructed with RAxML 7.2.8 and visualized with Dendroscope 3.2.10 (13). In every case, 1,000 bootstrap repetitions gave values higher than 900 for most branches. Pseudomonas fluorescens PfO-1 was used as the outgroup.
FIG 2
FIG 2
Peak biomarkers allowing MALDI-TOF MS to identify ST111, ST175, ST235, ST253, and ST395. The mass spectra were visualized with ClinProTools 3.0 (Bruker Daltonik). Units on the x axis represent the mass per charge (m/z), and those of the y axes represent the relative intensity in arbitrary units. (A) Peak at m/z 9,617 was specific to the STs of cluster 1, while that at m/z 9,586 was specific to the other STs. (B) Within cluster 1, isolates of ST235 specifically displayed a peak at m/z 11,610 and lacked a peak at m/z 5,813. (C) Isolates of ST111 were characterized by two specific peaks at m/z 3,657 and m/z 10,538 and the absence of a peak at m/z 12,579. (D) Absence of peaks at m/z 7,329 and m/z 12,154 combined with a peak at m/z 7,359 with a high intensity were specific to the ST175 isolates. (E) Isolates of ST253 lacked a specific peak at m/z 5,813. (F) Peak at m/z 8,550 was specific to isolates of ST395.
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