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. 2015 Jan 22:5:478.
doi: 10.3389/fgene.2014.00478. eCollection 2014.

HLA-B allele and haplotype diversity among Thai patients identified by PCR-SSOP: evidence for high risk of drug-induced hypersensitivity

Apichaya Puangpetch  1 , Napatrupron Koomdee  1 , Montri Chamnanphol  1 , Thawinee Jantararoungtong  1 , Siwalee Santon  1 , Santirhat Prommas  1 , Yaowaluck Hongkaew  1 , Chonlaphat Sukasem  1
Affiliations

HLA-B allele and haplotype diversity among Thai patients identified by PCR-SSOP: evidence for high risk of drug-induced hypersensitivity

Apichaya Puangpetch et al. Front Genet. .

Abstract

Background: There are 3 classes of HLA molecules; HLA class I, II and III, of which different classes have different functions. HLA-B gene which belongs to HLA class I play an important role predicting drug hypersensitivity.

Materials and methods: Nine hundred and eighty-six Thai subjects who registered at a pharmacogenomics laboratory were determined for HLA-B genotype using a two-stage sequence-specific oligonucleotide probe system (PCR-SSOP).

Results: In this study, HLA-B alleles did not deviate from Hardy-Weinberg equilibrium (P > 0.05). The most common HLA-B alleles observed in this population were HLA-B (*) 46:01 (11.51%), HLA-B (*) 58:01 (8.62%), HLA-B (*) 40:01 (8.22%), HLA-B (*) 15:02 (8.16%) and HLA-B (*) 13:01 (6.95%). This finding revealed that HLA-B allele frequency in the Thai population was consistent with the Chinese population (p > 0.05), however, differed from the Malaysian population (p < 0.05). The top five HLA-B genotypes were HLA-B (*) 40:01/46:01 (2.13%), HLA-B (*) 46:01/46:01 (2.03%), HLA-B (*) 40:01/58:01 (2.03%), HLA-B (*) 46:01/58:01 (1.93%) and HLA-B (*) 15:02/46:01 (1.83%). This study found that 15.92% of Thai subjects carry HLA-B (*) 15:02, which has been associated with carbamazepine-induced severe cutaneous adverse drug reactions (SCARs). Moreover, 16.33% of Thai subjects carry the HLA-B (*) 58:01 allele, which has been associated with allopurinol-induced SCARs.

Conclusion: This study demonstrates a high diversity of HLA-B polymorphisms in this Thai population. The high frequency of HLA-B pharmacogenomic markers in the population emphasizes the importance of such screening to predict/avoid drug hypersensitivity.

Keywords: HLA-B; PCR-SSOP; SCARs; Thai; drug hypersensitivity; pharmacogenomic markers.

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Figures

Figure 1
Figure 1
“Pharmacogenomics cards” have been successfully implemented in clinical practice in Thailand. The patients are tested for the HLA-B alleles which are associated with the ADRs related with the use of concerned drugs.
See this image and copyright information in PMC

References

    1. Alper C. A., Larsen C. E., Dubey D. P., Awdeh Z. L., Fici D. A., Yunis E. J. (2006). The haplotype structure of the human major histocompatibility complex. Hum. Immunol. 67, 73–84. 10.1016/j.humimm.2005.11.006 - DOI - PubMed
    1. Avila-Rios S., Ormsby C. E., Carlson J. M., Valenzuela-Ponce H., Blanco-Heredia J., Garrido-Rodriguez D., et al. (2009). Unique features of HLA-mediated HIV evolution in a Mexican cohort: a comparative study. Retrovirology 6:72. 10.1186/1742-4690-6-72 - DOI - PMC - PubMed
    1. Chantarangsu S., Mushiroda T., Mahasirimongkol S., Kiertiburanakul S., Sungkanuparph S., Manosuthi W., et al. (2009). HLA-B*3505 allele is a strong predictor for nevirapine-induced skin adverse drug reactions in HIV-infected Thai patients. Pharmacogenet. Genomics 19, 139–146. 10.1097/FPC.0b013e32831d0faf - DOI - PubMed
    1. Chantarangsu S., Mushiroda T., Mahasirimongkol S., Kiertiburanakul S., Sungkanuparph S., Manosuthi W., et al. (2011). Genome-wide association study identifies variations in 6p21.3 associated with nevirapine-induced rash. Clin. Infect. Dis. 53, 341–348. 10.1093/cid/cir403 - DOI - PubMed
    1. Chen P., Lin J. J., Lu C. S., Ong C. T., Hsieh P. F., Yang C. C., et al. (2011). Carbamazepine-induced toxic effects and HLA-B*1502 screening in Taiwan. N. Engl. J. Med. 364, 1126–1133. 10.1056/NEJMoa1009717 - DOI - PubMed