Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Jun 25;7(18):1370-7.

Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells

Jinghui Zheng  1 , Jian Liang  1 , Xin Deng  1 , Xiaofeng Chen  1 , Fasheng Wu  1 , Xiaofang Zhao  1 , Yuan Luo  1 , Lei Fu  1 , Zuling Jiang  1
Affiliations

Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells

Jinghui Zheng et al. Neural Regen Res. .

Abstract

Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

Keywords: Buyang Huanwu decoction; bone marrow mesenchymal stem cells; cell signal transduction pathway; extracellular signal-regulated protein kinase; mitogen-activated protein kinase signaling pathway; nestin; neural regeneration; neuron specific enolase.

PubMed Disclaimer

Conflict of interest statement

Conflicts of interest: None declared.

Figures

Figure 1
Figure 1
Rat bone marrow mesenchymal stem cells at primary, second and third passages (inverted phase contrast microscopy, × 100). (A) Primary cells were round. (B) Passage 2 cells formed colonies. (C) Passage 3 cells had a fusiform or fibrillar arrangement.
Figure 2
Figure 2
CD11b, CD45 and CD90 expression in rat bone marrow mesenchymal stem cells detected by flow cytometry. (A) Blank control cells. (B-D) FITC labeled CD90, CD11 and CD45 positive cells respectively. FITC: Fluorescein isothiocyanate.
Figure 3
Figure 3
Electropherogram of phosphorylated extracellular signal-regulated protein kinase (p-ERK) protein expression in rat bone marrow mesenchymal stem cells induced with Buyang Huanwu decoction (BYHWD) detected by western blot analysis. 1: Blank control group; 2: negative control group; 3: apr-BYHWD group. In the apr-BYHWD group, phosphorylated extracellular signal-regulated protein kinase protein expression peaked at day 28. apr-BYHWD: Active principle region of BYHWD.
Figure 4
Figure 4
Electropherogram of p38 phosphorylated protein (p-p38) expression in rat bone marrow mesenchymal stem cells induced with Buyang Huanwu decoction (BYHWD) detected by western blot analysis. 1: Blank control group; 2: negative control group; 3: apr-BYHWD group. In the apr-BYHWD group, p38 phosphorylated protein expression peaked at day 28. apr-BYHWD: Active principle region of BYHWD.
Figure 5
Figure 5
Reverse transcription-PCR electropherogram of neuron specific enolase mRNA expression after the mitogen-activated protein kinase pathway was blocked. 1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group; M: Marker; BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
Figure 6
Figure 6
Western blot electropherogram of neuron specific enolase expression after the mitogen-activated protein kinase pathway was blocked. 1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group; BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
Figure 7
Figure 7
Reverse transcription-PCR electropherogram of nestin mRNA expression after the mitogen-activated protein kinase pathway was blocked. 1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group; M: Marker; BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
Figure 8
Figure 8
Western blot electropherogram of nestin protein expression after the mitogen-activated protein kinase pathway was blocked. 1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group. BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Liu Y, Lin R, Shi X, et al. The roles of buyang huanwu decoction in anti-inflammation, antioxidation and regulation of lipid metabolism in rats with myocardial ischemia. Evid Based Complement Alternat Med 2011. 2011 561396. - PMC - PubMed
    1. Zhao YN, Wu XG, Li JM, et al. Effect of BuYangHuanWu recipe on cerebral microcirculation in gerbils with ischemia-reperfusion. Sichuan Da Xue Xue Bao Yi Xue Ban. 2010;41(1):53–56. - PubMed
    1. Wang WR, Lin R, Zhang H, et al. The effects of Buyang Huanwu Decoction on hemorheological disorders and energy metabolism in rats with coronary heart disease. J Ethnopharmacol. 2011;137(1):214–220. - PubMed
    1. Wang L, Jiang DM. Neuroprotective effect of Buyang Huanwu Decoction on spinal ischemia/reperfusion injury in rats. J Ethnopharmacol. 2009;124(2):219–223. - PubMed
    1. Trzaska KA, Rameshwar P. Dopaminergic neuronal differentiation protocol for human mesenchymal stem cells. Methods Mol Biol. 2011;698:295–303. - PubMed

LinkOut - more resources