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. 2015 Jan-Mar;4(1):13-8.
doi: 10.4103/2278-0203.149610.

Evaluation of the cytotoxicity of fiber reinforced composite bonded retainers and flexible spiral wires retainers in simulated high and low cariogenic environments

Arezoo Jahanbin  1 , Mostafa Shahabi  1 , Farzaneh Ahrari  1 , Yasaman Bozorgnia  2 , Arezoo Shajiei  3 , Hooman Shafaee  1 , Jalil Tavakkol Afshari  4
Affiliations

Evaluation of the cytotoxicity of fiber reinforced composite bonded retainers and flexible spiral wires retainers in simulated high and low cariogenic environments

Arezoo Jahanbin et al. J Orthod Sci. 2015 Jan-Mar.

Abstract

Objectives: The aim of this study was to evaluate the cytotoxic effects of fiber reinforced composite bonded retainers in comparison with flexible spiral wires (FSWs) under high and low cariogenic-simulated environments using human oral fibroblasts.

Materials and methods: Four types of bonded retainers were evaluated: (1) reinforced with glass fibers: Interlig (Angelus), (2) reinforced with polyethylene fibers: Connect (Kerr), (3) reinforced with quartz fibers: Quartz Splint UD (RTD), and (4) FSW. Twenty specimens of each sample group were prepared with the same surface area and halved. Next, half of them were placed in a high cariogenic environment 60 min in 10% lactic acid 3 times a day and remained in Fusayama Meyer artificial saliva for the rest of the day) and the other half were placed in a low cariogenic environment 20 min in 10% lactic acid 3 times a day and remained in Fusayama Meyer artificial saliva for the rest of the day) for 1, 7 and 30 days. Cell viability was assessed by MTT assay. Data were analyzed using SPSS software (α =0.05).

Results: During the 1(st) month, cytotoxicity reduced gradually. In the low cariogenic-simulated environment, the cytotoxicity of all of the groups were reported to be mild at day 30 and the difference between them was significant (P = 0.016). In the same period in the high cariogenic-simulated environment, the cytotoxicity of Connect and Quartz Splint was mild, and they had lower cytotoxicity than the other groups. Meanwhile, Interlig had moderate (52%) and FSW had severe cytotoxicity (22%) and the difference between the groups was also significant (P = 0.000).

Conclusions: FSW retainers are not recommended in those at high-risk for dental caries. However, in those at low-risk, there is no difference from the standpoint of cytotoxicity.

Keywords: Cell viability; cytotoxicity; fiber reinforced composite; retainer.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Cell viability in the Connect group,(a) High acidic environment,(b) Low acidic environment
Figure 2
Figure 2
Cell viability in the Interlig group, (a) High acidic environment, (b) Low acidic environment
Figure 3
Figure 3
Cell viability in the Quartz Splint group, (a) High acidic environment, (b) Low acidic environment
Figure 4
Figure 4
Cell viability in the Wire group, (a) High acidic environment, (b) Low acidic environment
Figure 5
Figure 5
Box plot for evaluating cell viability in the high acidic environment on day 7
See this image and copyright information in PMC

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