Comment on

• High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.

  Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung JK, Sander JD. Fu Y, et al. Nat Biotechnol. 2013 Sep;31(9):822-6. doi: 10.1038/nbt.2623. Epub 2013 Jun 23. Nat Biotechnol. 2013. PMID: 23792628 Free PMC article.
• Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases.

  Frock RL, Hu J, Meyers RM, Ho YJ, Kii E, Alt FW. Frock RL, et al. Nat Biotechnol. 2015 Feb;33(2):179-86. doi: 10.1038/nbt.3101. Epub 2014 Dec 15. Nat Biotechnol. 2015. PMID: 25503383 Free PMC article.
• GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases.

  Tsai SQ, Zheng Z, Nguyen NT, Liebers M, Topkar VV, Thapar V, Wyvekens N, Khayter C, Iafrate AJ, Le LP, Aryee MJ, Joung JK. Tsai SQ, et al. Nat Biotechnol. 2015 Feb;33(2):187-197. doi: 10.1038/nbt.3117. Epub 2014 Dec 16. Nat Biotechnol. 2015. PMID: 25513782 Free PMC article.
• Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors.

  Wang X, Wang Y, Wu X, Wang J, Wang Y, Qiu Z, Chang T, Huang H, Lin RJ, Yee JK. Wang X, et al. Nat Biotechnol. 2015 Feb;33(2):175-8. doi: 10.1038/nbt.3127. Epub 2015 Jan 19. Nat Biotechnol. 2015. PMID: 25599175