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. 2015 Apr;114(4):1611-2.
doi: 10.1007/s00436-015-4346-x. Epub 2015 Feb 8.

A cultivation method for growing bloodstream forms of Trypanosoma brucei to higher cell density and for longer time

Chinenye Ajoko  1 , Dietmar Steverding
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A cultivation method for growing bloodstream forms of Trypanosoma brucei to higher cell density and for longer time

Chinenye Ajoko et al. Parasitol Res. 2015 Apr.

Abstract

A novel cultivation technique using transwells allowing the growth of bloodstream forms of Trypanosoma brucei for longer periods of time and to higher cell densities is described. Trypanosomes cultured in transwells placed in cups of tissue culture plates containing sixfold excess of medium grew within 4 days to maximum cell densities of 2 × 10(7) parasites per milliliter. Compared to control cultures, the exponential growth of trypanosomes was 1 day longer and the maximum cell concentration was increased fourfold. The new culture system may be useful in drug screening assays and analysing the T. brucei secretome.

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