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. 2015 Jan-Apr;5(1):12-7.
doi: 10.4103/2229-516X.149222.

Effect of aminoguanidine on cardiovascular responses and survival time during blood loss: A study in normotensive and deoxycorticosterone acetate-salt hypertensive rats

Babak Barmaki  1 , Majid Khazaei  2
Affiliations

Effect of aminoguanidine on cardiovascular responses and survival time during blood loss: A study in normotensive and deoxycorticosterone acetate-salt hypertensive rats

Babak Barmaki et al. Int J Appl Basic Med Res. 2015 Jan-Apr.

Abstract

Introduction: Hemorrhagic shock causes more circulatory disturbances and mortality in hypertensive than normotensive subjects. In the late phase of hemorrhagic shock, nitric oxide (NO) overproduction leads to vascular decompensation. In this study, we evaluated the effect of inducible NO synthase (iNOS) inhibitor, aminoguanidine (AG), on hemodynamic parameters and serum nitrite concentration in decompensated hemorrhagic shock model in normotensive and hypertensive male rats.

Materials and methods: Twenty-four male rats were divided into hypertensive and normotensive groups (n = 12 each). Hypertension was induced by subcutaneous injection of deoxycorticoesterone acetate (DOCA), 30 mg/kg in uninephrectomized rats. Decompensated hemorrhagic shock was induced by withdrawing blood until the mean arterial pressure (MAP) reached 40 mmHg. After 120 min, each group was assigned to aminguanidine (100 mg/kg) and control group. Hemodynamic parameters were monitored for next 60 min. Blood samples were taken before and after shock period and 60 min after treatment. Survival rate was monitored for 72 h.

Results: Infusion of AG in normotensive animals caused a transient increase in MAP and increase of heart rate, whereas it did not affect those parameters in hypertensive animals. Hemorrhagic shock caused a significant rise in serum nitrite concentration in normotensive and hypertensive rats and infusion of AG did not significantly change it in both groups. No significant differences observed in survival rate between AG-treated and not treated groups.

Conclusion: It seems that inhibition of iNOS with AG does not have beneficial effects on hemodynamatic parameters and survival rate during decompensated hemorrhagic shock in normotensive and hypertensive animals.

Keywords: Hemorrhagic shock; hypertension; nitric oxide.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Changes of mean arterial pressure (a) and Heart rate (b) during the shock period in normotensive and hypertensive rats (a). *Significant difference compare to before experiment; #Significant difference compare to normotensive group.
Figure 2
Figure 2
Effect of aminoguanidine on mean arterial pressure and heart rate in normotensive (a and c) and hypertensive (b and d) rats
Figure 3
Figure 3
Comparison of serum nitrite concentrations during the shock period and after aminoguanidine (AG) treatment in normotensive and hypertensive groups. *P < 0.05 compare to base level. #P < 0.05 compare to AG-treated group
Figure 4
Figure 4
Survival rate of normotensive and hypertensive animals aftershock period in aminoguanidine-treated and nontreated groups
See this image and copyright information in PMC

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