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. 2015 Mar;22(3):269-71.
doi: 10.1038/nsmb.2963. Epub 2015 Feb 9.

Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex

Christopher H S Aylett  1 , Daniel Boehringer  1 , Jan P Erzberger  1 , Tanja Schaefer  1 , Nenad Ban  1
Affiliations

Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex

Christopher H S Aylett et al. Nat Struct Mol Biol. 2015 Mar.

Abstract

Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.

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References

    1. J Struct Biol. 1999 Dec 1;128(1):82-97 - PubMed
    1. PLoS One. 2012;7(7):e40464 - PubMed
    1. Mol Cell Biol. 2004 Nov;24(21):9437-55 - PubMed
    1. Nat Protoc. 2009;4(3):363-71 - PubMed
    1. Mol Cell Proteomics. 2010 Aug;9(8):1634-49 - PubMed

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