Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Dec 8;45(3):213-26.
doi: 10.1007/BF00507696.

A histochemical study of the apparent deamination of proteins by sodium hypochlorite

P J Stoward

A histochemical study of the apparent deamination of proteins by sodium hypochlorite

P J Stoward. Histochemistry. .

Abstract

The possible chemical mechanisms by which neutral solutions of sodium hypochlorite containing a high concentration of sodium chloride abolish the acidophilia of proteins in sections of fixed tissue are reviewed. The most probable one is the chlorination of the protein terminal amino groups, followed by the breakdown of the N-chloramine so formed into alpha-ketocarboxylic acid, nitrile or aldehyde groups. Hypochlorite solutions certainly do not deaminate tissue sections as was previously thought. Experimental evidence for the formation of relatively stable N-chloramine groups in situ and their limited conversion to aldehydes is reported. For example, the acidophilia of hypochlorite-treated sections was found to be restored after flooding them with hydriodic acid followed by the extraction of the liberated iodine with an alcohol. The significance of these experimental findings is discussed.

PubMed Disclaimer

References

    1. J R Microsc Soc. 1967;87(1):77-103 - PubMed
    1. Histochemie. 1968;14(2):212-4 - PubMed
    1. Tissue Cell. 1970;2(1):155-79 - PubMed
    1. J R Microsc Soc. 1967;87(2):247-57 - PubMed
    1. J Cell Sci. 1971 May;8(3):709-25 - PubMed