In silico-prediction of downstream MYB interacting partners of MAPK3 in Arabidopsis

Abstract

Protein-Protein interactions (PPIs) are vital to most biological processes thus the identification of PPIs is of primary importance. Here, we endeavor to identify the downstream interacting partners of (AtMAPK3P) in Arabidopsis thaliana using the docking approach. Out of 131 members of MYB transcription factors 41 members are showing interactions with AtMAPK3P while the rest are showing non-interaction. Using minimal sequence motif search as well as through docking approach several novel MYB interacting proteins were also reported in the present study which need to be confirmed by in vitro kinase assay. Together, the results obtained essentially enhance our knowledge of the MAPK interacting protein network and provide a valuable research resource for developing a nearly important link between pathogen-activated MAPK signaling pathways and downstream transcriptional programming.

Keywords: MAPK; MYB; Protein -Protein interactions (PPIs); Transcription factor (TF); docking.