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. 2015 Jan;79(1):16-21.

Cross-protection against Salmonella Typhimurium infection conferred by a live attenuated Salmonella Enteritidis vaccine

Rahul M Nandre  1 , Dajeong Lee  1 , John Hwa Lee  1
Affiliations

Cross-protection against Salmonella Typhimurium infection conferred by a live attenuated Salmonella Enteritidis vaccine

Rahul M Nandre et al. Can J Vet Res. 2015 Jan.

Abstract
in English, French

In this study, a genetically engineered live attenuated Salmonella Enteritidis (SE) vaccine was evaluated for its ability to protect against Salmonella Typhimurium (ST) infection in chickens. The birds were orally primed with the vaccine on the 1st day of life and given an oral booster at 5 wk of age. Control birds were orally inoculated with phosphate-buffered saline. Both groups of birds were orally challenged with a virulent ST strain at 9 wk of age. Compared with the control chickens, the vaccinated chickens had significantly higher levels of systemic IgG and mucosal IgA against specific ST antigens and a significantly greater lymphoproliferative response to ST antigens. The excretion of ST into the feces was significantly lower in the vaccinated group than in the control group on days 9 and 13 d after challenge. In addition, the vaccinated group had significantly fewer pronounced gross lesions in the liver and spleen and lower bacterial counts in the internal organs than the control group after challenge. These data indicate that genetically engineered live attenuated SE may induce humoral and cellular immune responses against ST antigens and may confer protection against virulent ST challenge.

Dans la présente étude on évalua la capacité d’un vaccin vivant atténué génétiquement modifié de Salmonella Enteritidis (SE) à protéger contre une infection par Salmonella Typhimurium (ST) chez le poulet. Les poulets furent inoculés oralement avec le vaccin à leur premier jour de vie et reçurent un rappel à 5 semaines d’âge. Les oiseaux témoins reçurent par voie orale de la saline tamponnée. Les deux groupes furent challengés par voie orale avec une souche virulente de ST à 9 sem d’âge. Comparativement aux oiseaux témoins, les poulets vaccinés avaient des taux significativement plus élevés d’IgG systémiques et d’IgA locaux contre des antigènes spécifiques de ST et une réponse lympho-proliférative significativement plus importante aux antigènes de ST. L’excrétion de ST dans les fèces était significativement moindre dans le groupe vacciné comparativement au groupe témoin aux jours 9 et 13 après le challenge. De plus, après le challenge le groupe vacciné avait significativement moins de lésions macroscopiques marquées dans le foie et la rate et des dénombrements bactériens moindres dans les organes internes que le groupe témoin. Ces résultats indiquent que le vaccin SE vivant génétiquement modifié peut induire une réponse immunitaire humorale et cellulaire contre des antigènes de ST et peut conférer une protection contre un challenge avec une souche de ST virulente.(Traduit par Docteur Serge Messier).

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Figures

Figure 1
Figure 1
Mean levels [± standard deviation (SD)] of plasma IgG against antigens specific to Salmonella Typhimurium (ST) in chickens vaccinated on day 1 of life with the genetically engineered live attenuated Salmonella Enteritidis (SE) JOL919. The arrow indicates booster vaccination. The asterisks indicate significant differences (P < 0.05) between the values in the vaccinated group (black bars) and those in the control group (white bars).
Figure 2
Figure 2
Mean levels (± SD) of secretory IgA (sIgA) against ST-specific antigens measured in intestinal lavage samples from chickens vaccinated with JOL919. The arrow, asterisks, and bars have the same meanings as in Figure 1.
Figure 3
Figure 3
Lymphocyte-stimulation responses against ST-specific antigens, expressed as the mean stimulation index (± SD). The asterisks and bars have the same meanings as in Figure 1.
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