Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Dec;25(4):425-9.
doi: 10.1007/s13337-014-0226-8. Epub 2014 Aug 31.

Isolation and characterization of a nodavirus associated with mass mortality in Asian seabass (Lates calcarifer) from the west coast of India

Debashis Banerjee  1 , Mohammed A Hamod  2 , Thangavel Suresh  1 , Indrani Karunasagar  1
Affiliations

Isolation and characterization of a nodavirus associated with mass mortality in Asian seabass (Lates calcarifer) from the west coast of India

Debashis Banerjee et al. Virusdisease. 2014 Dec.

Abstract

A fish nodavirus was detected in the juveniles of Asian seabass (Lates calcarifer) during a massive outbreak in the seabass cage culture farm located in the south west coast of India. The clinical signs of the disease included anorexia, inflated abdomen, exophthalmia, darkening of the whole body, erratic swimming and cork-screw type movement followed by death. The dead and the moribund fish were analyzed for nodavirus by reverse transcriptase-polymerase chain reaction (RT-PCR) using specific primers targeting the T4 region of RNA2 coat protein gene. This is the first report of nodavirus infection in the fresh water cage-reared seabass fish in the west coast of India. The piscine nodavirus was detected in the brain, retina and kidney of all the fishes examined. The PCR products were cloned and sequenced. The sequence analysis showed more than 90 % homology with the other coat protein gene sequence of piscine nodaviruses from other countries. The phylogenetic analysis based on the partial nucleotide sequence of RNA2 coat protein gene revealed that the virus belonged to the red-spotted grouper nervous necrosis virus, which is one of the widely distributed genotype among the other four known genotypes of piscine nodavirus.

Keywords: Coat protein gene (RNA2); Lates calcarifer; Nodavirus; Reverse transcription PCR (RT-PCR); Viral nervous necrosis (VNN).

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Detection of fish nodavirus by reverse transcriptase PCR. A T4NV-F2/R primer; M: 100 bp DNA ladder, Genei, Bangalore, Lane 1: nodavirus positive control, Lane 2: negative control, Lanes 3–6: nodavirus positive samples. B LCNV-F/R primer; M: 100 bp DNA ladder, Lane 1: nodavirus positive control, Lane 2: negative control, Lanes 3–6: nodavirus positive samples
Fig. 2
Fig. 2
Phylogenetic tree deduced from the variable region of coat protein sequences of 23 betanodaviruses. HM017076 and HM017077 are the T4 coat protein partial gene sequences generated in this study
See this image and copyright information in PMC

References

    1. Arimoto M, Mushiake K, Mizuta Y, Nakai T, Muroga K, Furusawa I. Detection of striped jack nervous necrosis virus (SJNNV) by enzyme-linked immunosorbent assay (ELISA) Fish Pathol. 1992;27:191–195. doi: 10.3147/jsfp.27.191. - DOI
    1. Azad IS, Shekar MS, Thirunavukkarasu AR, Poornima M, Kailasan M, Rajan JJS, Ali SA, Abraham M, Ravichandran P. Nodavirus infection causes mortalities in hatchery produced larvae of Lates calcarifer: first report from India. Dis Aquat Organ. 2005;63:113–118. doi: 10.3354/dao063113. - DOI - PubMed
    1. Azad IS, Jithedran KP, Shekhara MS, Thirunavukkarasua AR, de Lapena LD. Immunolocalization of nervous necrosis virus indicates vertical transmission in hatchery produced Asian seabass (Lates calcraifer Bloch)—a case study. Aquaculture. 2006;255:39–47. doi: 10.1016/j.aquaculture.2005.04.076. - DOI
    1. Breuil B, Pepin JFP, Boscher S, Thiery R. Experimental vertical transmission of nodavirus from broodfish to eggs and larvae of the seabass, Dicentrarchus labrax (L.) J Fish Dis. 2002;25:697–702. doi: 10.1046/j.1365-2761.2002.00406.x. - DOI
    1. Comps M, Trindade M, Delsert CL. Investigation of encephalitis viruses (FEV) expression in marine fishes using DIG-labelled probes. Aquaculture. 1996;143:113–121. doi: 10.1016/0044-8486(96)01264-1. - DOI

LinkOut - more resources