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. 1989 Jul;171(7):4067-70.
doi: 10.1128/jb.171.7.4067-4070.1989.

Isolation of nit-4, the minor nitrogen regulatory gene which mediates nitrate induction in Neurospora crassa

Y H Fu  1 , J Y Kneesi, G A Marzluf
Affiliations

Isolation of nit-4, the minor nitrogen regulatory gene which mediates nitrate induction in Neurospora crassa

Y H Fu et al. J Bacteriol. 1989 Jul.

Abstract

Expression of nitrate reductase in Neurospora crassa requires the positive action of nit-4, a pathway-specific regulatory gene, which mediates nitrate induction. We report the molecular cloning of the nit-4 gene and present results which suggest that the nit-4 gene is constitutively expressed to yield a low-abundance 2.2-kilobase transcript. These results indicate that the nit-2 major control gene and the nit-4 pathway-specific control gene independently regulate the expression of the nitrate assimilatory structural genes.

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