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. 2015 May 14;51(39):8245-8.
doi: 10.1039/c4cc07910h.

Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

Rasa Rakauskaitė  1 , Giedrė Urbanavičiūtė, Audronė Rukšėnaitė, Zita Liutkevičiūtė, Robertas Juškėnas, Viktoras Masevičius, Saulius Klimašauskas
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Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

Rasa Rakauskaitė et al. Chem Commun (Camb). .

Abstract

Selenocysteine is a valuable component of both natural selenoproteins and designer biocatalysts; however the availability of such proteins is hampered by technical limitations. Here we report the first general strategy for the production of selenoproteins via genetically-encoded incorporation of a synthetic photocaged selenocysteine residue in yeast cells, and provide examples of light-controlled protein dimerization and targeted covalent labeling in vitro.

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