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. 2015 Mar 1:6:1-9.
doi: 10.1016/j.euprot.2014.11.002.

The Serum Immunoglobulin G Glycosylation Signature of Gastric Cancer

L Renee Ruhaak  1 , Donald A Barkauskas  2 , Javier Torres  3 , Cara L Cooke  4 , Lauren D Wu  1 , Carol Stroble  1 , Sureyya Ozcan  1 , Cynthia C Williams  1 , Margarita Camorlinga  3 , David M Rocke  5 , Carlito B Lebrilla  6 , Jay V Solnick  4
Affiliations

The Serum Immunoglobulin G Glycosylation Signature of Gastric Cancer

L Renee Ruhaak et al. EuPA Open Proteom. .

Abstract

Biomarkers may facilitate detection of gastric cancer at an earlier stage and reduce mortality. Here we sought to determine if the glycosylation profile of serum immunoglobulin G (IgG) could distinguish patients with non-atrophic gastritis (NAG), duodenal ulcer (DU) and gastric cancer (GC). Serum IgG was released and analyzed using nano-LC-TOF mass spectrometry. Statistically significant false discovery rate (FDR)-adjusted p-values were observed for 18 glycans, eight that differed significantly between NAG and GC, three that distinguished NAG from DU, and eight that differed between DU and GC. The IgG glycosylation signature may be useful as a predictive marker for gastric cancer.

Keywords: Gastric cancer; N-glycan; duodenal ulcer; gastritis; nanoLC-MS; serum.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1. Glycan groups show altered abundances with gastric cancer
Bars represent the mean (± SEM) relative abundance of the glycan features in each group. Glycan features for a given group do not sum to 100% because there are glycans that have more than a single feature. For example, if a glycan is fucosylated and sialyated, it is counted is both subgroups. Significant differences (denoted by *) were found among the diagnostic groups for truncated, biantennary, and sialyated glycans with FDR-adjusted p-values of 0.021, 0.017 and 0.039, respectively
Figure 2
Figure 2. Levels of Individual glycans differ with disease state
Mean difference as well as 95% confidence interval is displayed for glycans that differ significantly between (A) NAG versus DU (black) and NAG versus GC (grey), and (B) DU versus GC. Levels of glycans are significantly altered when the 95% C.I. does not include 0. Positive values indicate increased levels, while negative values indicate decreased levels of a certain glycan. X-axis labels represent the glycan composition (reading left to right): number of hexoses, N-acetylhexosamines, fucoses, and sialic acids. For example 3_4_1_0 indicates 3 hexoses, 4 N-acetylhexosamines, 1 fucose, and 0 sialic acids.
Figure 3
Figure 3. The altered IgG glycosylation profile that is associated with gastric cancer
Increasing glycans are shown on the left, while decreasing glycans are shown on the right. Symbol key: blue square: N-acetyl glucosamine, green circle: mannose, yellow circle: galactose, red triangle: fucose and purple diamond: sialic acid.
See this image and copyright information in PMC

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