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. 2015:2015:718180.
doi: 10.1155/2015/718180. Epub 2015 Jan 15.

Effects of TGF-β1 on OPG/RANKL expression of cementoblasts and osteoblasts are similar without stress but different with mechanical compressive stress

Xianrui Yang  1 , Yanmin Wang  1 , Xianglong Han  1 , Rui Shu  1 , Tian Chen  1 , Huan Zeng  1 , Xin Xu  1 , Lan Huang  2 , Aishu Ren  2 , Jinlin Song  2 , Li Cao  2 , Ding Bai  1
Affiliations

Effects of TGF-β1 on OPG/RANKL expression of cementoblasts and osteoblasts are similar without stress but different with mechanical compressive stress

Xianrui Yang et al. ScientificWorldJournal. 2015.

Abstract

Introduction: This study aimed to explore the effects of TGF-β1 on regulating activities of cementoblasts and osteoblasts with or without stress.

Material and methods: Human recombinant TGF-β1 was added with different doses. Immunohistochemical test of osteoprotegerin (OPG)/receptor activator of nuclear factor-kappaB ligand (RANKL) and Alizarin Red-S staining were conducted. Mechanical compressive stress was obtained by increasing the pressure of gaseous phase. OPG/RANKL expression was detected in both cells through quantitative real-time PCR.

Results: Similar significant differences (P < 0.05) existed in OPG/RANKL change with increasing concentration of TGF-β1 without mechanical stress for cementoblasts and osteoblasts. However, under 3 h stress, OPG increased and RANKL decreased significantly (P < 0.01) but with similar OPG/RANKL change. Moreover, under 24 h stress, OPG change exhibited no difference (P > 0.05), but RANKL decreased significantly (P < 0.01) at 10 and 100 ng/mL TGF-β1 in cementoblasts. In osteoblasts, OPG increased significantly (P < 0.01) at 10 and 100 ng/mL, whereas RANKL decreased with statistical difference (P < 0.05) at 1 and 10 ng/mL.

Conclusions: The effects of TGF-β1 on OPG/RANKL expression of cementoblasts and osteoblasts are similar even without mechanical stress. However, these effects are different under mechanical compressive stress.

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Figures

Figure 1
Figure 1
Morphological observation of cementoblasts and osteoblasts under microscope.
Figure 2
Figure 2
Immunohistochemical results of OPG expression in OCCM-30 and MC3T3-E1 cells with TGF-β1 treatment.
Figure 3
Figure 3
Immunohistochemical results of RANKL expression in OCCM-30 and MC3T3-E1 cells with TGF-β1 treatment.
Figure 4
Figure 4
Alizarin Red-S staining results of mineralization in OCCM-30 and MC3T3-E1 cells with TGF-β1 treatment.
Figure 5
Figure 5
Effects of TGF-β1 on cementoblasts and osteoblasts without mechanical stress.
Figure 6
Figure 6
Effects of TGF-β1 on cementoblasts and osteoblasts under 3 h mechanical compressive stress.
Figure 7
Figure 7
Effects of TGF-β1 on cementoblasts and osteoblasts under 24 h mechanical compressive stress.
Figure 8
Figure 8
Change tendencies of OPG and RANKL under different mechanical compressive stress durations.
See this image and copyright information in PMC

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