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. 2015:2015:698310.
doi: 10.1155/2015/698310. Epub 2015 Jan 22.

Salivary desmoglein enzyme-linked immunosorbent assay for diagnosis of pemphigus vulgaris: a noninvasive alternative test to serum assessment

Hossein Mortazavi  1 , Alireza Khatami  2 , Zahra Seyedin  3 , Iman Vasheghani Farahani  4 , Maryam Daneshpazhooh  1
Affiliations

Salivary desmoglein enzyme-linked immunosorbent assay for diagnosis of pemphigus vulgaris: a noninvasive alternative test to serum assessment

Hossein Mortazavi et al. Biomed Res Int. 2015.

Abstract

Background: Serum desmoglein enzyme-linked immunosorbent assay (ELISA) is used for the diagnosis and monitoring of pemphigus diseases.

Objectives: To compare the diagnostic accuracy of salivary antidesmoglein (Dsg) 1 and 3 ELISA in the diagnosis of pemphigus vulgaris (PV) patients with that of serum desmogleins ELISA.

Methods: Eighty-six untreated PV patients and 180 age- and sex-matched PV-free controls were recruited in this case-control study. PV was diagnosed based on clinical, histopathological, and direct immunofluorescence findings. After processing, serum and salivary anti-Dsg 1 and 3 were measured by the ELISA method using Euroimmun kit (Lübeck, Germany).

Results: Using the cut-off point of 20 relative units (RU)/mL, the serum anti-Dsg 1 and 3 ELISA were positive in 62 (72.1%) and 83 (96.5%) patients, respectively, and the salivary anti-Dsg 1 and 3 ELISA were positive in 31 (36.1%) and 63 (73.3%) patients, respectively. The specificity of salivary anti-Dsg 1 and anti-Dsg 3 were both 98.9%. Optimal cut-off values of 7.7 and 13.4 RU/mL were determined for the salivary anti-Dsg 1 and anti-Dsg 3 ELISA, respectively.

Conclusion: Salivary anti-Dsg 1 and 3 ELISA with high specificities (98.9%) could be suggested as safe and noninvasive methods for the diagnosis of PV when obtaining a blood sample is difficult.

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Figures

Figure 1
Figure 1
The ROC curve drawn to obtain the optimal cut-off value for salivary (a) and serum (b) Dsg 1 and 3 ELISA.
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References

    1. Amagai M., Komai A., Hashimoto T., et al. Usefulness of enzyme-linked immunosorbent assay using recombinant desmogleins 1 and 3 for serodiagnosis of pemphigus. British Journal of Dermatology. 1999;140(2):351–357. doi: 10.1046/j.1365-2133.1999.02752.x. - DOI - PubMed
    1. Tanikawa A., Amagai M. Pemphigus treatment in Japan. Dermatologic Clinics. 2011;29(4):685–686. doi: 10.1016/j.det.2011.07.004. - DOI - PubMed
    1. Mortazavi H., Shahdi M., Amirzargar A. A., et al. Desmoglein ELISA in the diagnosis of pemphigus and its correlation with the severity of pemphigus vulgaris. Iranian Journal of Allergy, Asthma and Immunology. 2009;8(1):53–56. - PubMed
    1. Lee Y.-H., Wong D. T. Saliva: an emerging biofluid for early detection of diseases. American Journal of Dentistry. 2009;22(4):241–248. - PMC - PubMed
    1. Hallaji Z., Mortazavi H., Lajevardi V., et al. Serum and salivary desmoglein 1 and 3 enzyme-linked immunosorbent assay in pemphigus vulgaris: correlation with phenotype and severity. Journal of the European Academy of Dermatology and Venereology. 2010;24(3):275–280. doi: 10.1111/j.1468-3083.2009.03408.x. - DOI - PubMed

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