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. 2015 Feb 18;10(2):e0117950.
doi: 10.1371/journal.pone.0117950. eCollection 2015.

Multi locus variable-number tandem repeat (MLVA) typing tools improved the surveillance of Salmonella enteritidis: a 6 years retrospective study

Sophie Bertrand  1 , Guillaume De Lamine de Bex  1 , Christa Wildemauwe  1 , Octavie Lunguya  2 , Marie France Phoba  2 , Benedikt Ley  3 , Jan Jacobs  4 , Raymond Vanhoof  1 , Wesley Mattheus  1
Affiliations

Multi locus variable-number tandem repeat (MLVA) typing tools improved the surveillance of Salmonella enteritidis: a 6 years retrospective study

Sophie Bertrand et al. PLoS One. .

Erratum in

Abstract

Surveillance of Salmonella enterica subsp. enterica serovar Enteritidis is generally considered to benefit from molecular techniques like multiple-locus variable-number of tandem repeats analysis (MLVA), which allow early detection and confinement of outbreaks. Here, a surveillance study, including phage typing, antimicrobial susceptibility testing and MLVA on 1,535 S. Enteritidis isolates collected between 2007 and 2012, was used to evaluate the added value of MLVA for public health surveillance in Belgium. Phage types PT4, PT8, PT21, PT1, PT6, PT14b, PT28 and PT13 dominate the Belgian S. Enteritidis population. The isolates of S. Enteritidis were most frequently susceptible to all antibiotics tested. 172 different MLVA profiles were detected, of which 9 frequent profiles included 67.2% of the S. Enteritidis population. During a serial passage experiment on selected isolates to investigate the in vitro stability of the 5 MLVA loci, no variations over time were observed indicating that the MLVA profiles were stable. The MLVA profile of isolates originating from different outbreaks in the Democratic Republic of the Congo (DRC) between 2010 and 2011 were distinct from any of the MLVA profiles found in Belgian isolates throughout the six year observational period and demonstrates that MLVA improves public health surveillance of S. Enteritidis. However, MLVA should be complemented with other subtyping methods when investigating outbreaks is caused by the most common MLVA profile.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Phage type distribution among the S. Enteritidis population over the 6-year period (n- = 1,498; other: all phage types with less than 3% occurrence in the same combined population.
Fig 2
Fig 2. a: Annual distribution of Belgian S. Enteritidis isolates over the 6-year period according to the nine most frequent MLVA profiles (representing 1,006 of the 1,498; 67.2%).
b: Prevalence of S. Enteritidis isolates in this study with the most frequent MLVA profile and with indication of the major phage type (representing 902 of the 1,498; 60.2%).
Fig 3
Fig 3. Minimal spanning tree calculated of MLVA profiles of S. Enteritidis isolates over the 6-year period.
Each node represents a different MLVA profile with frequency-dependent size. Node colour represents phage type: Branch thickness indicates how many loci are different in the MLVA profiles of the connected nodes. Thick solid lines connect nodes that differ by one MLVA locus, thin solid lines connect nodes that differ by two MLVA loci and dashed lines connect nodes that differ by three MLVA loci.
See this image and copyright information in PMC

References

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