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. 2015 May;53(5):1719-21.
doi: 10.1128/JCM.02358-14. Epub 2015 Feb 18.

Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis

Denis Filisetti  1 , Hélène Yera  2 , Odile Villard  3 , Benoît Escande  4 , Estelle Wafo  5 , Véronique Houfflin-Debarge  6 , Laurence Delhaes  7 , Patrick Bastien  8
Affiliations

Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis

Denis Filisetti et al. J Clin Microbiol. 2015 May.

Abstract

We evaluated the molecular diagnosis of congenital toxoplasmosis (CT) on neonatal amniotic fluid samples from 488 mother-child pairs. Maternal infection during pregnancy was diagnosed and dated or could not be ruled out. Forty-six cases of CT were defined according to the European Research Network on CT classification system and case definitions. Neonatal amniotic fluid testing had an overall sensitivity of 54% (95% confidence interval [95% CI], 39 to 69%) and a specificity of 100% (95% CI, 99 to 100%). Its sensitivity was 33% (95% CI, 13 to 59%) when antenatal diagnosis was positive and 68% (95% CI, 48 to 84%) when antenatal diagnosis was negative or lacking. This difference in sensitivity may have been due to treatment of antenatally diagnosed cases. Relative to postnatal serology, neonatal amniotic fluid testing allowed an earlier diagnosis to be made in 26% of the cases (95% CI, 9 to 51%).

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