Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 May;53(5):1725-6.
doi: 10.1128/JCM.03691-14. Epub 2015 Feb 18.

A new real-time reverse transcription-PCR assay for detection of human enterovirus 68 in respiratory samples

Antonio Piralla  1 , Alessia Girello  1 , Marta Premoli  1 , Fausto Baldanti  2
Affiliations

A new real-time reverse transcription-PCR assay for detection of human enterovirus 68 in respiratory samples

Antonio Piralla et al. J Clin Microbiol. 2015 May.

Abstract

A global reemergence of human enterovirus 68 (EV-D68) associated with severe respiratory illness occurred in 2014. We developed and validated an EV-D68-specific real-time reverse transcription-PCR (RT-PCR) for the detection of EV-D68 in respiratory samples. The rapid diagnosis of EV-D68 may contribute to better management of EV-D68 infections.

PubMed Disclaimer

Figures

FIG 1
FIG 1
(A) Linear range for the EV-D68 real-time RT-PCR assay. The standard curve was obtained by plotting the CT values against the starting copy number. The coefficient R2 of linear regression is shown. (B) Probit regression analysis to determine the limit of detection (LOD) for the EV-D68-specific real-time RT-PCR. TheLOD with a 95% probability is represented by a gray dotted line.
See this image and copyright information in PMC

References

    1. Oberste MS, Maher K, Schnurr D, Flemister MR, Lovchik JC, Peters H, Sessions W, Kirk C, Chatterjee N, Fuller S, Hanauer JM, Pallansch MA. 2004. Enterovirus 68 is associated with respiratory illness and shares biological features with both the enteroviruses and the rhinoviruses. J Gen Virol 85:2577–2584. doi:10.1099/vir.0.79925-0. - DOI - PubMed
    1. Centers for Disease Control and Prevention. 2011. Clusters of acute respiratory illness associated with human enterovirus 68—Asia, Europe, and United States, 2008–2010. MMWR Morb Mortal Wkly Rep 60:1301–1304. - PubMed
    1. Meijer A, Benschop K, Donker G, van der Avoort H. 2014. Continued seasonal circulation of enterovirus D68 in the Netherlands, 2011–2014. Euro Surveill 19:20935 http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20935. - PubMed
    1. Piralla A, Girello A, Grignani M, Gozalo-Margüello M, Marchi A, Marseglia G, Baldanti F. 2014. Phylogenetic characterization of enterovirus 68 strains in patients with respiratory syndromes in Italy. J Med Virol 86:1590–1593. doi:10.1002/jmv.23821. - DOI - PMC - PubMed
    1. Piralla A, Lilleri D, Sarasini A, Marchi A, Zecca M, Stronati M, Baldanti F, Gerna G. 2012. Human rhinovirus and human respiratory enterovirus (EV68 and EV104) infections in hospitalized patients in Italy, 2008–2009. Diagn Microbiol Infect Dis 73:162–167. doi:10.1016/j.diagmicrobio.2012.02.019. - DOI - PubMed

Publication types

MeSH terms

LinkOut - more resources