Improvement of acetic acid tolerance of Saccharomyces cerevisiae using a zinc-finger-based artificial transcription factor and identification of novel genes involved in acetic acid tolerance
- PMID: 25698512
- DOI: 10.1007/s00253-014-6343-x
Improvement of acetic acid tolerance of Saccharomyces cerevisiae using a zinc-finger-based artificial transcription factor and identification of novel genes involved in acetic acid tolerance
Abstract
Acetic acid is present in cellulosic hydrolysate as a potent inhibitor, and the superior acetic acid tolerance of Saccharomyces cerevisiae ensures good cell viability and efficient ethanol production when cellulosic raw materials are used as substrates. In this study, a mutant strain of S. cerevisiae ATCC4126 (Sc4126-M01) with improved acetic acid tolerance was obtained through screening strains transformed with an artificial zinc finger protein transcription factor (ZFP-TF) library. Further analysis indicated that improved acetic acid tolerance was associated with improved catalase (CAT) activity. The ZFP coding sequence associated with the improved phenotype was identified, and real-time RT-PCR analysis revealed that three of the possible genes involved in the enhanced acetic acid tolerance regulated by this ZFP-TF, namely YFL040W, QDR3, and IKS1, showed decreased transcription levels in Sc4126-M01 in the presence of acetic acid, compared to those in the control strain. Sc4126-M01 mutants having QDR3 and IKS1 deletion (ΔQDR3 and ΔIKS1) exhibited higher acetic acid tolerance than the wild-type strain under acetic acid treatment. Glucose consumption rate and ethanol productivity in the presence of 5 g/L acetic acid were improved in the ΔQDR3 mutant compared to the wild-type strain. Our studies demonstrated that the synthetic ZFP-TF library can be used to improve acetic acid tolerance of S. cerevisiae and that the employment of an artificial transcription factor can facilitate the exploration of novel functional genes involved in stress tolerance of S. cerevisiae.
Similar articles
-
Absence of Rtt109p, a fungal-specific histone acetyltransferase, results in improved acetic acid tolerance of Saccharomyces cerevisiae.FEMS Yeast Res. 2016 Mar;16(2):fow010. doi: 10.1093/femsyr/fow010. Epub 2016 Feb 5. FEMS Yeast Res. 2016. PMID: 26851403
-
Improved growth and ethanol fermentation of Saccharomyces cerevisiae in the presence of acetic acid by overexpression of SET5 and PPR1.Biotechnol J. 2015 Dec;10(12):1903-11. doi: 10.1002/biot.201500508. Epub 2015 Nov 11. Biotechnol J. 2015. PMID: 26479519
-
[Breeding of robust industrial ethanol-tolerant Saccharomyces cerevisiae strain by artificial zinc finger protein library].Sheng Wu Gong Cheng Xue Bao. 2013 May;29(5):612-9. Sheng Wu Gong Cheng Xue Bao. 2013. PMID: 24010359 Chinese.
-
Omics analysis of acetic acid tolerance in Saccharomyces cerevisiae.World J Microbiol Biotechnol. 2017 May;33(5):94. doi: 10.1007/s11274-017-2259-9. Epub 2017 Apr 12. World J Microbiol Biotechnol. 2017. PMID: 28405910 Review.
-
[Advances in functional genomics studies underlying acetic acid tolerance of Saccharomyces cerevisiae].Sheng Wu Gong Cheng Xue Bao. 2014 Mar;30(3):368-80. Sheng Wu Gong Cheng Xue Bao. 2014. PMID: 25007573 Review. Chinese.
Cited by
-
A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations.Biotechnol Biofuels. 2016 Aug 12;9:173. doi: 10.1186/s13068-016-0583-1. eCollection 2016. Biotechnol Biofuels. 2016. PMID: 27525042 Free PMC article.
-
Physiological and Molecular Characterization of an Oxidative Stress-Resistant Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering.Front Microbiol. 2022 Feb 24;13:822864. doi: 10.3389/fmicb.2022.822864. eCollection 2022. Front Microbiol. 2022. PMID: 35283819 Free PMC article.
-
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance.Synth Syst Biotechnol. 2024 May 29;9(4):723-732. doi: 10.1016/j.synbio.2024.05.013. eCollection 2024 Dec. Synth Syst Biotechnol. 2024. PMID: 38882181 Free PMC article.
-
Inactivation of the transcription factor mig1 (YGL035C) in Saccharomyces cerevisiae improves tolerance towards monocarboxylic weak acids: acetic, formic and levulinic acid.J Ind Microbiol Biotechnol. 2018 Aug;45(8):735-751. doi: 10.1007/s10295-018-2053-1. Epub 2018 Jun 6. J Ind Microbiol Biotechnol. 2018. PMID: 29876685
-
Implementing principles of traditional concentrated grape must fermentation to the production of new generation balsamic vinegars. Starter selection and effectiveness.J Food Sci Technol. 2016 Sep;53(9):3424-3436. doi: 10.1007/s13197-016-2306-9. Epub 2016 Oct 5. J Food Sci Technol. 2016. PMID: 27777448 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous