Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Mar;39(1):27-32.
doi: 10.1007/s12639-013-0276-7. Epub 2013 Mar 9.

Genetic characterization of Fasciola gigantica from different geographical regions of India by ribosomal DNA markers

O K Raina  1 , Siju Susan Jacob  1 , M Sankar  1 , D Bhattacharya  2 , S Bandyopadyay  3 , Anju Varghese  4 , Jayanta Kumar Chamuah  1 , H Lalrinkima  1
Affiliations

Genetic characterization of Fasciola gigantica from different geographical regions of India by ribosomal DNA markers

O K Raina et al. J Parasit Dis. 2015 Mar.

Abstract

Ribosomal DNA sequences of the second internal transcribed spacer (ITS-2) and 28S ribosomal DNA (618 bp) of Fasciola gigantica collected from cattle and buffaloes from four different geographical locations of India, were characterized for genotyping. ITS-2 sequence was analyzed in 28 worms that was typical of F. gigantica and differed at six positions, with one of these being a distinguishing deletion (T) at the 327th position in F. gigantica relative to F. hepatica. However, Fasciola specimens also showed intraspecies sequence polymorphism in the ITS-2, with two different ITS-2 sequences existing in the ribosomal DNA (rDNA) array within a single Fasciola worm. One of the sequences was identical to that of F. gigantica and the other showed extensive sequence polymorphism in the ITS-2. Using BspH1-restriction fragment length polymorphism, six variable ITS-2 sequences in F. gigantica were identified within these parasite specimens and were found distributed in these four geographical regions. 28S rDNA sequence of 24 flukes, collected from the above four geographical regions, showed a single nucleotide polymorphism at 284th nucleotide (G/A). Analyzing the sequence data of 28S rDNA of F. gigantica available from some African and Asian countries for this polymorphic 284th nucleotide position, it is proposed that there are two basic lineages of the F. gigantica for 28S rDNA existing in the fluke populations from five African and several Asian countries.

Keywords: 28S ribosomal DNA; Fasciola gigantica; Genetic characterization; ITS-2.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Map of India showing the places where Fasciola specimens from cattle and buffaloes were collected and used in this study
Fig. 2
Fig. 2
BspH1 digestion of ITS-2 PCR product of four Indian isolates of F. gigantica; Srinagar (lane1), Bangalore (lane 2), Izatnagar (lane 3) and Kolkata (lane 4). Lane M 100 bp ladder DNA marker and UN undigested ITS-2 product
Fig. 3
Fig. 3
Evolutionary relationship of 28S rDNA partial sequence of F. gigantica isolates of India (Srinagar, Bangalore, Kolkata and Izatnagar) with different isolates of Iran, Thailand and Africa (17 taxa) and one taxon of out group (28S ribosomal RNA sequence of Dicrocoelium spp.) established using maximum likelihood method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) is shown above the branches
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Agatsuma T, Arakawa Y, Iwagami M, Honzako Y, Cahyaningsih U, Kang SY, Hong SJ. Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica. Parasitol Int. 2000;49:231–238. doi: 10.1016/S1383-5769(00)00051-9. - DOI - PubMed
    1. Ali H, Ai L, Song HQ, Ali S, Lin RQ, Seyni B, Issa G, Zhu XQ. Genetic characterization of Fasciola samples from different host species and geographical localities revealed the existence of F. hepatica and F. gigantica in Niger. Parasitol Res. 2008;102:1021–1024. doi: 10.1007/s00436-007-0870-7. - DOI - PubMed
    1. Ashrafi K, Valero MA, Panova M, Periago MV, Massoud J, Mas-Coma S. Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran. Parasitol Int. 2006;55:249–260. doi: 10.1016/j.parint.2006.06.003. - DOI - PubMed
    1. Douady CJ, Delsuc F, Boucher Y, Doolittle WF, Douzer EJ. Comparison of Bayesian and maximum likelihood bootstrap measures of phylogenetic reliability. Mol Biol Evol. 2003;20:248–254. doi: 10.1093/molbev/msg042. - DOI - PubMed
    1. Gunasekar KR, Tewari AK, Sreekumar C, Gupta SC, Rao JR. Elucidation of genetic variability among different isolates of Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA-polymerase chain reaction. Parasitol Res. 2008;103:1075–1081. doi: 10.1007/s00436-008-1095-0. - DOI - PubMed

LinkOut - more resources