Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989;106(3-4):341-5.
doi: 10.1007/BF01313963.

Detection of HIV-1 DNA in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. Rapid communication

F T Hufert  1 , D von Laer, C Schramm, A Tárnok, H Schmitz
Affiliations

Detection of HIV-1 DNA in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. Rapid communication

F T Hufert et al. Arch Virol. 1989.

Abstract

The presence of HIV-1 proviral DNA was determined in CD4 cells, CD8 cells and macrophages/monocytes obtained from peripheral blood of 8 HIV-1 infected persons. Using the polymerase chain reaction (PCR) we were able to detect proviral DNA in the extracts of only 10(2)-10(3) CD4 (T4) cells. In contrast, 10(5) CD8 cells did not contain detectable amounts of proviral DNA. Surprisingly, in four of our eight patients studied no HIV-1 DNA was found in macrophages. In peripheral blood, every hundred T4 cell may be infected with HIV-1.

PubMed Disclaimer

References

    1. J Infect Dis. 1988 Dec;158(6):1158-69 - PubMed
    1. Cytometry. 1988 Jul;9(4):309-15 - PubMed
    1. Science. 1984 Jul 6;225(4657):59-63 - PubMed
    1. Lancet. 1988 Sep 10;2(8611):596-9 - PubMed
    1. J Clin Microbiol. 1988 Jul;26(7):1416-8 - PubMed

MeSH terms