Current trends in magnetic particle enrichment for mass spectrometry-based analysis of cardiovascular protein biomarkers

Abstract

Magnetic particles have traditionally been utilized to isolate and enrich various cardiovascular protein biomarkers for mass spectrometry-based proteomic analysis. The application of functionalized magnetic particles for immunocapture is attractive due to their easy manipulation, large surface area-to-volume ratios for maximal antibody binding, good recovery and high magnetic saturation. Magnetic particle enrichment coupled with mass spectrometry can act as a complementary tool for clinical sandwich-immunoassay development since it can provide improved target specificity and true metrological traceability. The purpose of this review is to summarize current separation methods and technologies that use magnetic particles to enrich protein biomarkers from complex matrices, specifically focusing on cardiovascular disease-related proteins and the advantages of magnetic particles over existing techniques.

Keywords: LC-MS/MS; bioseparation; cardiac protein biomarkers; cardiovascular disease; enrichment; immunoassay; magnetic (micro) beads; magnetic nanoparticles; mass spectrometry.