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. 2015:2015:642652.
doi: 10.1155/2015/642652. Epub 2015 Feb 1.

Detection of human cytomegalovirus in different histopathological types of glioma in Iraqi patients

Haidar A Shamran  1 , Haider S Kadhim  2 , Aws R Hussain  3 , Abdulameer Kareem  4 , Dennis D Taub  5 , Robert L Price  6 , Mitzi Nagarkatti  7 , Prakash S Nagarkatti  7 , Udai P Singh  7
Affiliations

Detection of human cytomegalovirus in different histopathological types of glioma in Iraqi patients

Haidar A Shamran et al. Biomed Res Int. 2015.

Abstract

Human Cytomegalovirus (HCMV) is an endemic herpes virus that reemerges in cancer patients enhancing oncogenic potential. HCMV infection is associated with certain types of cancer morbidity such as glioblastomas. HCMV, like all other herpes viruses, has the ability to remain latent within the body of the host and can contribute in chronic inflammation. To determine the role of HCMV in glioma pathogenesis, paraffin-embedded blocks from glioma patients (n = 50) and from benign meningioma patients (n = 30) were obtained and evaluated by immunohistochemistry and polymerase chain reaction for the evidence of HCMV antigen expression and the presence of viral DNA. We detected HCMV antigen and DNA for IEI-72, pp65, and late antigen in 33/36, 28/36, and 26/36 in glioblastoma multiforme patients whereas 12/14, 10/14, and 9/14 in anaplastic astrocytoma patients, respectively. Furthermore, 84% of glioma patients were positive for immunoglobulin G (IgG) compared to 72.5% among control samples (P = 0.04). These data indicate the presence of the HCMV virus in a high percentage of glioma samples demonstrating distinct histopathological grades and support previous reports showing the presence of HCMV infection in glioma tissue. These studies demonstrate that detection of low-levels of latent viral infections may play an active role in glioma development and pathogenesis.

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Figures

Figure 1
Figure 1
Immunohistochemical staining of HCMV in glioma. (a) Immunohistochemical stain of glioma section with IE1-72 protein. (b) Control without secondary antibody. (c) Immunohistochemical stains of glioma section with pp65 protein. (d) Control without secondary antibody. (e) Immunohistochemical staining of glioma with late antigen antibody. (f) Control without secondary antibody. (g) Lung known positive control stain for HCMV.
Figure 2
Figure 2
Detection of HCMV DNA in malignant glioma tissue (nested PCR products) visualized with UV light M: 1000 bp DNA marker. Lanes 1–10: viral DNA amplified with nested PCR. Lane 11: negative result. The size of the PCR product is 144 bp.
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