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. 2015:2015:823829.
doi: 10.1155/2015/823829. Epub 2015 Feb 1.

In vitro inhibitory and cytotoxic activity of MFM 501, a novel codonopsinine derivative, against Methicillin-Resistant Staphylococcus aureus clinical isolates

Saiful Azmi Johari  1 , Mastura Mohtar  2 , Sharifah Aminah Syed Mohammad  3 , Rohana Sahdan  4 , Zurina Shaameri  5 , Ahmad Sazali Hamzah  5 , Mohd Fazli Mohammat  5
Affiliations

In vitro inhibitory and cytotoxic activity of MFM 501, a novel codonopsinine derivative, against Methicillin-Resistant Staphylococcus aureus clinical isolates

Saiful Azmi Johari et al. Biomed Res Int. 2015.

Abstract

28 new pyrrolidine types of compounds as analogues for natural polyhydroxy alkaloids of codonopsinine were evaluated for their anti-MRSA activity using MIC and MBC value determination assay against a panel of S. aureus isolates. One pyrrolidine compound, MFM 501, exhibited good inhibitory activity with MIC value of 15.6 to 31.3 μg/mL against 55 S. aureus isolates (43 MRSA and 12 MSSA isolates). The active compound also displayed MBC values between 250 and 500 μg/mL against 58 S. aureus isolates (45 MRSA and 13 MSSA isolates) implying that MFM 501 has a bacteriostatic rather than bactericidal effect against both MRSA and MSSA isolates. In addition, MFM 501 showed no apparent cytotoxicity activity towards three normal cell lines (WRL-68, Vero, and 3T3) with IC50 values of >625 µg/mL. Selectivity index (SI) of MFM 501 gave a value of >10 suggesting that MFM 501 is significant and suitable for further in vivo investigations. These results suggested that synthetically derived intermediate compounds based on natural products may play an important role in the discovery of new anti-infective agents against MRSA.

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Figures

Figure 1
Figure 1
List of the 28 synthetically produced codonopsinine derivatives used in this study.
Figure 2
Figure 2
Chemical structure of codonopsinine.
See this image and copyright information in PMC

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References

    1. Sameerah S. A. R. Pattern of antibiotic usage in MOH hospitals. Proceedings of the New Strategies in Combating Anti-Infectives Resistance Seminar; October 2009; Melaka, Malaysia. Keynote;
    1. Lailatul A. Introduction: antibiotic resistance in Malaysia. Keynote Presentation at the New Strategies in Combating Anti- Infectives Resistance Seminar; October 2009; Melaka, Malaysia;
    1. Hughes A. J., Ariffin N., Huat T. L., et al. Prevalence of nosocomial infection and antibiotic use at a university medical center in Malaysia. Infection Control and Hospital Epidemiology. 2005;26(1):100–104. doi: 10.1086/502494. - DOI - PubMed
    1. Ramli S. R., Neoh H.-M., Aziz M. N., Hussin S. Screening and detection of heterogenous vancomycin intermediate Staphylococcus aureus in Hospital Kuala Lumpur Malaysia, using the glycopeptide resistance detection Etest and population analysis profiling. Infectious Disease Reports. 2012;4(1):71–72. doi: 10.4081/idr.2012.e20. - DOI - PMC - PubMed
    1. Ahmad N., Ling L. N., Ghani M. K. A., Nawi S. The presence of heterogeneous vancomycin-intermediate Staphylococcus aureus (heteroVISA) in a major Malaysian hospital. Medical Journal of Malaysia. 2012;67(3):269–273. - PubMed

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