Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures

doi:10.1007/978-1-4939-2438-7_8

. 2015:1282:73-87.

doi: 10.1007/978-1-4939-2438-7_8.

Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures

Hulda R Jonsdottir¹, Ronald Dijkman

Affiliations

PMID: 25720473
PMCID: PMC7124022
DOI: 10.1007/978-1-4939-2438-7_8

Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures

Hulda R Jonsdottir et al. Methods Mol Biol. 2015.

. 2015:1282:73-87.

doi: 10.1007/978-1-4939-2438-7_8.

Authors

Hulda R Jonsdottir¹, Ronald Dijkman

Affiliation

¹ Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, 3012, Switzerland.

PMID: 25720473
PMCID: PMC7124022
DOI: 10.1007/978-1-4939-2438-7_8

Abstract

The human airway serves as the entry point of human respiratory viruses, including human coronaviruses. In this chapter we outline the methods by which we establish fully differentiated airway epithelium and its use for human coronavirus propagation. Additionally, we outline methods for immunofluorescence staining of these cultures for virus detection, characterization of cell tropism, and how to perform antiviral assays and quantify viral replication.

PubMed Disclaimer

Cited by

In vitro virucidal activity of Echinaforce®, an Echinacea purpurea preparation, against coronaviruses, including common cold coronavirus 229E and SARS-CoV-2.
Signer J, Jonsdottir HR, Albrich WC, Strasser M, Züst R, Ryter S, Ackermann-Gäumann R, Lenz N, Siegrist D, Suter A, Schoop R, Engler OB. Signer J, et al. Virol J. 2020 Sep 9;17(1):136. doi: 10.1186/s12985-020-01401-2. Virol J. 2020. PMID: 32907596 Free PMC article.
The IFNL4 Gene Is a Noncanonical Interferon Gene with a Unique but Evolutionarily Conserved Regulation.
Zhou H, Møhlenberg M, Terczyńska-Dyla E, Winther KG, Hansen NH, Vad-Nielsen J, Laloli L, Dijkman R, Nielsen AL, Gad HH, Hartmann R. Zhou H, et al. J Virol. 2020 Feb 14;94(5):e01535-19. doi: 10.1128/JVI.01535-19. Print 2020 Feb 14. J Virol. 2020. PMID: 31776283 Free PMC article.
Differential interferon-α subtype induced immune signatures are associated with suppression of SARS-CoV-2 infection.
Schuhenn J, Meister TL, Todt D, Bracht T, Schork K, Billaud JN, Elsner C, Heinen N, Karakoese Z, Haid S, Kumar S, Brunotte L, Eisenacher M, Di Y, Lew J, Falzarano D, Chen J, Yuan Z, Pietschmann T, Wiegmann B, Uebner H, Taube C, Le-Trilling VTK, Trilling M, Krawczyk A, Ludwig S, Sitek B, Steinmann E, Dittmer U, Lavender KJ, Sutter K, Pfaender S. Schuhenn J, et al. Proc Natl Acad Sci U S A. 2022 Feb 22;119(8):e2111600119. doi: 10.1073/pnas.2111600119. Proc Natl Acad Sci U S A. 2022. PMID: 35131898 Free PMC article.
A clinical protocol for a German birth cohort study of the Maturation of Immunity Against respiratory viral Infections (MIAI).
Hartmann CR, Khan R, Schöning J, Richter M, Willers M, Pirr S, Heckmann J, Dirks J, Morbach H, Konrad M, Fries E, Winkler M, Büchel J, Seidenspinner S, Fischer J, Vollmuth C, Meinhardt M, Marissen J, Schmolke M, Haid S, Pietschmann T, Backes S, Dölken L, Löber U, Keil T, Heuschmann PU, Wöckel A, Sagar, Ulas T, Forslund-Startceva SK, Härtel C, Viemann D. Hartmann CR, et al. Front Immunol. 2024 Sep 17;15:1443665. doi: 10.3389/fimmu.2024.1443665. eCollection 2024. Front Immunol. 2024. PMID: 39355253 Free PMC article.
Determining the Replication Kinetics and Cellular Tropism of Influenza D Virus on Primary Well-Differentiated Human Airway Epithelial Cells.
Holwerda M, Kelly J, Laloli L, Stürmer I, Portmann J, Stalder H, Dijkman R. Holwerda M, et al. Viruses. 2019 Apr 24;11(4):377. doi: 10.3390/v11040377. Viruses. 2019. PMID: 31022887 Free PMC article.

See all "Cited by" articles

References

1. de Jong PM, van Sterkenburg MA, Hesseling SC, et al. Ciliogenesis in human bronchial epithelial cells cultured at the air-liquid interface. Am J Respir Cell Mol Biol. 1994;10(3):271–277. doi: 10.1165/ajrcmb.10.3.8117445. - DOI - PubMed
1. Lin H, Li H, Cho HJ, et al. Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies. J Pharm Sci. 2007;96(2):341–350. doi: 10.1002/jps.20803. - DOI - PubMed
1. Fuchs S, Hollins AJ, Laue M, et al. Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C. Cell Tissue Res. 2003;311(1):31–45. doi: 10.1007/s00441-002-0653-5. - DOI - PubMed
1. Pyrc K, Sims AC, Dijkman R, et al. Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures. J Virol. 2010;84(21):11255–11263. doi: 10.1128/JVI.00947-10. - DOI - PMC - PubMed
1. Kindler E, Jonsdottir HR, Muth D, et al. Efficient replication of the novel human betacoronavirus EMC on primary human epithelium highlights its zoonotic potential. MBio. 2013;4(1):e00611–e00612. doi: 10.1128/mBio.00611-12. - DOI - PMC - PubMed

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions

LinkOut - more resources

Full Text Sources
Other Literature Sources
- scite Smart Citations

[1] de Jong PM, van Sterkenburg MA, Hesseling SC, et al. Ciliogenesis in human bronchial epithelial cells cultured at the air-liquid interface. Am J Respir Cell Mol Biol. 1994;10(3):271–277. doi: 10.1165/ajrcmb.10.3.8117445. - DOI - PubMed

[2] de Jong PM, van Sterkenburg MA, Hesseling SC, et al. Ciliogenesis in human bronchial epithelial cells cultured at the air-liquid interface. Am J Respir Cell Mol Biol. 1994;10(3):271–277. doi: 10.1165/ajrcmb.10.3.8117445. - DOI - PubMed

[3] Lin H, Li H, Cho HJ, et al. Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies. J Pharm Sci. 2007;96(2):341–350. doi: 10.1002/jps.20803. - DOI - PubMed

[4] Lin H, Li H, Cho HJ, et al. Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies. J Pharm Sci. 2007;96(2):341–350. doi: 10.1002/jps.20803. - DOI - PubMed

[5] Fuchs S, Hollins AJ, Laue M, et al. Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C. Cell Tissue Res. 2003;311(1):31–45. doi: 10.1007/s00441-002-0653-5. - DOI - PubMed

[6] Fuchs S, Hollins AJ, Laue M, et al. Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C. Cell Tissue Res. 2003;311(1):31–45. doi: 10.1007/s00441-002-0653-5. - DOI - PubMed

[7] Pyrc K, Sims AC, Dijkman R, et al. Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures. J Virol. 2010;84(21):11255–11263. doi: 10.1128/JVI.00947-10. - DOI - PMC - PubMed

[8] Pyrc K, Sims AC, Dijkman R, et al. Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures. J Virol. 2010;84(21):11255–11263. doi: 10.1128/JVI.00947-10. - DOI - PMC - PubMed

[9] Kindler E, Jonsdottir HR, Muth D, et al. Efficient replication of the novel human betacoronavirus EMC on primary human epithelium highlights its zoonotic potential. MBio. 2013;4(1):e00611–e00612. doi: 10.1128/mBio.00611-12. - DOI - PMC - PubMed

[10] Kindler E, Jonsdottir HR, Muth D, et al. Efficient replication of the novel human betacoronavirus EMC on primary human epithelium highlights its zoonotic potential. MBio. 2013;4(1):e00611–e00612. doi: 10.1128/mBio.00611-12. - DOI - PMC - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures

Affiliation

Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures

Authors

Affiliation

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Other Literature Sources