Rapid determination of total aflatoxins and ochratoxins A in meat products by immuno-affinity fluorimetry

Abstract

Total aflatoxins (AFT) and ochratoxin A (OTA) levels were estimated using the VICAM AflaTest and OchraTest immunoaffinity fluorometric method in a total of 50 meat products (25 each of beef luncheon and beef burger) purchased from different supermarkets in Mansoura city, Egypt. All the meat samples analyzed were contaminated with both AFT and OTA with mean values of 1.1 μg/kg and 5.23 μg/kg, respectively, for beef luncheon and mean values of 3.22 μg/kg and 4.55 μg/kg, respectively, for beef burger. None of the beef luncheon and burger samples analyzed exceeded the permissible limits set by FDA for AFT, but 40% of beef burgers exceeded the FAO AFT permissible limit. Similarly, 52% and 36% of beef luncheon and beef burger samples exceeded the FAO OTA permissible limit. Application of the immunoaffinity fluorometric method is an accurate, safe and rapid method for mycotoxins determination in meat products to ensure their safety for human consumption.

Keywords: Aflatoxin B1 (CTD: 186907); Aflatoxin B2 (CID: 2724360); Aflatoxin G1 (CID: 14421); Aflatoxin G2 (CID: 2724362); Aflatoxin M1 (CID: 15558498); Aflatoxin M2 (CID: 23318); Aflatoxins; Beef; Immuno-affinity fluorimetry; Methanol (CID: 887); Ochratoxin A; Ochratoxin A (CID: 442530); Sodium chloride (CID: 5234); Tween 20 (CID: 443314).