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. 2015 Jun;70(6):829-34.
doi: 10.1007/s00284-015-0794-x. Epub 2015 Feb 28.

Phenotypic and genotypic characterization of biofilm formation in Staphylococcus haemolyticus

Elaine M Barros  1 , Moara Lemos, Thais Souto-Padrón, Marcia Giambiagi-deMarval
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Phenotypic and genotypic characterization of biofilm formation in Staphylococcus haemolyticus

Elaine M Barros et al. Curr Microbiol. 2015 Jun.

Abstract

Staphylococcus haemolyticus is one of the most frequently isolated coagulase-negative staphylococci. The ability to produce biofilm has contributed to its emergence as a nosocomial pathogen. In this study, some growth conditions were tested to determine their influence on biofilm formation. Brain-heart infusion (BHI) broth containing glucose was used to screen 64 clinical strains. A strong biofilm producer strain showed cells surrounded by a thick layer of extracellular matrix. The presence of atlE, fbp, bap, and icaA genes was analyzed. We concluded that S. haemolyticus biofilm production can be increased with cells grown in BHI, and highlighted that it could be an ica-independent process.

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