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. 2015 Mar;8(1):1-6.
doi: 10.3342/ceo.2015.8.1.1. Epub 2015 Feb 3.

Do all antioxidant supplements have the same potential effect on preventing myringosclerosis?

Seden Akdagli  1 , Arzu Tuzuner  2 , Sule Demirci  2 , Hatice Karadas  2 , Kamil Gökce Tulaci  2 , Mehmet Dogan  3 , Refik Caylan  4
Affiliations

Do all antioxidant supplements have the same potential effect on preventing myringosclerosis?

Seden Akdagli et al. Clin Exp Otorhinolaryngol. 2015 Mar.

Abstract

Objectives: Myringosclerosis is an irreversible pathological healing mechanism of the tympanic membrane which can result in the formation of sclerotic plaques. Antioxidant treatment is a recognised prevention therapy and coenzyme Q10 (CoQ10), lycopene, and grape seed extract (GSE), were used in this manner.

Methods: Forty-four Wistar rats were used in this experiment, and, following myringotomies, the animals were randomly divided into four groups. CoQ10, lycopene or GSE was administered orally to the respective groups, starting from the day of surgery. Otomicroscopy examination was performed on the 14th day. All tympanic membrane lesions were evaluated and compared otomicroscopically and histopathologically.

Results: The otomicroscopy and histopathological findings, compared against a control (saline) group, showed the CoQ10, lycopene, and GSE groups had statistically significant differences of degree of sclerosis (P<0.001).

Conclusion: CoQ10, lycopene, and GSE were compared against a saline group and their antioxidative and anti-inflammatory effects were similar. The formation of myringosclerotic plagues after experimental myringotomy in rats significantly decreased and diminished after systemic administration of the three different antioxidant supplements.

Keywords: Antioxidant; Coenzyme Q10; Grape Seed Extract; Lycopene; Myringosclerosis.

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Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1
Coenzyme Q10 group. There is no thickening of tympanic membrane and no fibrosis (arrow) (H&E, ×200).
Fig. 2
Fig. 2
Grape seed extract group. Grade 1 sclerosis, mild fibrotic change is highlighted with green color (arrow) (Masson's trichrome, ×200).
Fig. 3
Fig. 3
Lycopene group. Grade 2 sclerosis, moderate thickening of tympanic membrane and fibroblastic hyperplasia (arrows) (H&E, ×200).
Fig. 4
Fig. 4
Saline group. Grade 3 sclerosis, extensive thickening of tympanic membrane due to sclerotic changes in lamina propria (arrow) (H&E, ×100).
Fig. 5
Fig. 5
Trichrome stain of the same rat in Fig. 4 (saline group, grade 3 sclerosis) shows marked fibrosis in lamina propria (arrow) (Masson's trichrome, ×100).
Fig. 6
Fig. 6
Box-plot for sclerosis of tympanic membrane evaluation in histopathologic examination. Group 1, control group; Group 2, grape seed extract administered group; Group 3, coenzyme Q10 administered group; Group 4, lycopene administered group.
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