Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2015 Mar 1;37(3):498-504.
doi: 10.1016/j.clinthera.2015.02.004. Epub 2015 Feb 27.

Salivary diagnostics using a portable point-of-service platform: a review

Prarthana Khanna  1 , David R Walt  2
Affiliations
Review

Salivary diagnostics using a portable point-of-service platform: a review

Prarthana Khanna et al. Clin Ther. .

Abstract

Clinical diagnostics can be improved by faster and more accessible disease detection. Our laboratory has developed a point-of-service (POS) device capable of rapid, sensitive, automated, and multiplexed biomarker detection that uses human saliva instead of other biofluids. Here, we review the technology that led to the development of this POS device. This POS technology can advance clinical diagnostics by saving time because of faster diagnosis, saving money because of a shorter hospital stay, and ultimately improving clinical care.

Keywords: biomarker; detection; device; diagnostic; disease; saliva.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest statement

The authors PK and DW declare that no other relationships/conditions/circumstances present a potential conflict of interest with this submitted work. There were no study sponsors involved in the study design, in the collection, analysis and interpretation of data; in the writing of the manuscript; and in the decision to submit the manuscript for publication.

Figures

Figure 1
Figure 1. Point of service (POS) device, developed in our laboratory for salivary diagnostics, present at a patient’s hospital bedside
The device is powered by a conventional AC adapter providing 12 VDC at 8.5 A (not shown), and the case is 15 cm (wide) × 15 cm (tall) × 25 cm (deep). The weight of the device is 2.7 kg. The concentrations of multiple protein biomarkers in the saliva sample are quantified via sandwich immunoassays conducted on a microfluidic chip present in the device.
Figure 2
Figure 2. Workflow for the protein sandwich immunoassay
Microspheres are coupled with capture antibodies, incubated with a saliva sample and then biotinylated detection antibodies, and finally a streptavidin-conjugated fluorescent probe is added to the complex for a quantified readout.
Figure 3
Figure 3. Depiction of encoding beads with multiple fluorescent dyes to produce numerous bead types for multiplexed arrays
Microspheres are encoded with different concentrations of two fluorescent dyes, Coumarin (C30) and Europium (Eu-TTA) to produce distinct bead types to be used for detection of multiple biomarkers simultaneously.
See this image and copyright information in PMC

References

    1. Carpenter GH. The Secretion, Components, and Properties of Saliva. Annual Review of Food Science and Technology. 2013;4(1):267–76. - PubMed
    1. Wong DT. Salivary diagnostics powered by nanotechnologies, proteomics and genomics. J Am Dent Assoc. 2006;137(3):313–21. - PubMed
    1. Mandel I. The role of saliva in maintaining oral homeostasis. The Journal of the American Dental Association. 1989;119(2):298–304. - PubMed
    1. Humphrey SP, Williamson RT. A review of saliva: Normal composition, flow, and function. The Journal of Prosthetic Dentistry. 2001;85(2):162–9. - PubMed
    1. Mandel ID, Wotman S. The salivary secretions in health and disease. Oral sciences reviews. 1976;(8):25–47. - PubMed

LinkOut - more resources