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. 2014 Dec 1;7(12):e11152.
doi: 10.5812/jjm.11152. eCollection 2014 Dec.

Seminal Levels of Pro-inflammatory (CXCL1, CXCL9, CXCL10) and Homeostatic (CXCL12) Chemokines in Men With Asymptomatic Chlamydia trachomatis Infection

Hamid Hakimi  1 , Nahid Zainodini  1 , Hossein Khorramdelazad  2 , Mohammad Kazemi Arababadi  1 , Gholamhossein Hassanshahi  2
Affiliations

Seminal Levels of Pro-inflammatory (CXCL1, CXCL9, CXCL10) and Homeostatic (CXCL12) Chemokines in Men With Asymptomatic Chlamydia trachomatis Infection

Hamid Hakimi et al. Jundishapur J Microbiol. .

Abstract

Background: Chemokines play important roles in immune system activation against microbial infections.

Objectives: The current study aimed to evaluate seminal levels of CXC chemokines CXCL1, CXCL9, CXCL10 and CXCL12 in Chlamydia trachomatis infected patients.

Materials and methods: The C. trachomatis infection was determined employing Polymerase Chain Reaction (PCR)-based methods. Seminal concentrations of CXCL1, CXCL9, CXCL10 and CXCL12 were measured by Enzyme-Linked Immunosorbent Assay (ELISA).

Results: The current study results demonstrated that the semen levels of CXCL1 and CXCL9, but not CXCL10 and CXCL12, significantly increased in C. trachomatis infected patients compared to the healthy controls.

Conclusions: Based on the current study results, it may be concluded that both CXCL1 and CXCL9 play more important roles than CXCL10 and CXCL12 in induction of immune responses against C. trachomatis and could possibly be considered as future targets for immunotherapy of C. trachomatis infection.

Keywords: Chemokine, CXCL1, CXCL9, CXCL10 and CXCL12; Chlamydia trachomatis.

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Figures

Figure 1.
Figure 1.. PCR Amplification of Chlamydia trachomatis
The figure presents PCR amplification of C. trachomatis K/SotonK1 plasmid pSotonK1 (517 bp). Sample 1 (S1), S2 and S3 as well as S8 presents the negative samples and negative control, respectively. S4, S5 and S6 are positive samples and S9 is positive control.
Figure 2.
Figure 2.. Seminal Levels of CXCL1 in Men with Asymptomatic Chlamydia trachomatis Infection and Control
Figure 3.
Figure 3.. Seminal Levels of CXCL9 in Men with Asymptomatic Chlamydia trachomatis Infection and Control
Figure 4.
Figure 4.. Semen Levels of CXCL10 and CXCL12
The figure illustrates no significant increase of the semen levels of CXCL10 and CXCL12, patients infected with Chlamydia trachomatis, and the controls.
See this image and copyright information in PMC

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