Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Mar 15;7(8):589-94.
doi: 10.3969/j.issn.1673-5374.2012.08.005.

Association between p75 neurotrophin receptor gene expression and cell apoptosis in tissues surrounding hematomas in rat models of intracerebral hemorrhage

Baixiang He  1 , Gang Bao  1 , Shiwen Guo  1 , Gaofeng Xu  1 , Qi Li  1 , Ning Wang  1
Affiliations

Association between p75 neurotrophin receptor gene expression and cell apoptosis in tissues surrounding hematomas in rat models of intracerebral hemorrhage

Baixiang He et al. Neural Regen Res. .

Abstract

Animal models of intracerebral hemorrhage were established by injection of autologous blood into the caudate nucleus in rats. Cell apoptosis was measured by flow cytometry and immunohistochemical staining of the p75 neurotrophin receptor. p75 neurotrophin receptor protein was detected by immunohistochemistry. p75 neurotrophin receptor mRNA was examined by quantitative real-time polymerase chain reactions. At 24 hours after modeling, cellular apoptosis occured around hematoma with upregulation of p75 neurotrophin receptor protein and mRNA was observed, which directly correlated to apoptosis. This observation indicated that p75 neurotrophin receptor upregulation was associated with cell apoptosis around hematomas after intracerebral hemorrhage.

Keywords: apoptosis; intracerebral hemorrhage; neural regeneration; p75 neurotrophin receptor.

PubMed Disclaimer

Conflict of interest statement

Conflicts of interest: None declared.

Figures

Figure 1
Figure 1
Percentage of apoptosis at 6, 24, 72 hours, and 10 days after intracerebral hemorrhage, respectively as determined by flow cytometry. The apoptosis rate is presented in the tables in the lower right quadrants. FITC: Fluorescein isothiocyanate; PI: propidium iodide. (A–D) Left lower quadrant, annexin V-FITC-/PI- is the live cell population; lower right quadrant, annexin V-FITC+/PI- is the apoptotic cell population; upper right quadrant, annexin V-FITC+/PI+ is the necrotic cell population; left upper quadrant, annexin V-FITC-/PI+ is cell debris.
Figure 2
Figure 2
Hematoxylin-eosin (HE) and immunohistochemical staining of brain tissue surrounding hematomas after intracerebral hemorrhage (ICH). (A) The yellow arrow indicates neuronal apoptosis as shown by darkly stained cytoplasm and chromatin margination around the hematoma. The blue arrow shows the infiltration of neutrophils, suggesting that inflammation occurs around the hematoma after ICH (HE staining, × 400). (B) After ICH, brain tissue edema around the hematoma occurs as indicated by light staining and cell numbers decrease close to the hematoma, (HE staining, × 200). (C) Perivascular edema, suggesting vascular edema after ICH (HE staining, × 400). (D) p75 neurotrophin receptor is mainly expressed in the cytoplasm of neurons in the control (HE staining, × 400). (E–H) Immunohistochemical staining at 6, 24, 72 hours, and 10 days after ICH, respectively (× 400).
See this image and copyright information in PMC

References

    1. Kenchappa RS, Tep C, Korade Z, et al. p75 neurotrophin receptor-mediated apoptosis in sympathetic neurons involves a biphasic activation of JNK and up-regulation of tumor necrosis factor-alpha-converting enzyme/ADAM17. J Biol Chem. 2010;285(26):20358–20368. - PMC - PubMed
    1. Chen Y, Zeng J, Cen L, et al. Multiple roles of the p75 neurotrophin receptor in the nervous system. J Int Med Res. 2009;37(2):281–288. - PubMed
    1. Powers WJ. Intracerebral hemorrhage and head trauma: common effects and common mechanisms of injury. Stroke. 2010;41(10 Suppl):S107–110. - PMC - PubMed
    1. Zhang XQ, Zhang ZM, Yin XL, et al. Exploring the optimal operation time for patients with hypertensive intracerebral hemorrhage: tracking the expression and progress of cell apoptosis of prehematomal brain tissues. Chin Med J (Engl) 2010;123(10):1246–1250. - PubMed
    1. Chau PH, Woo J, Goggins WB, et al. Trends in stroke incidence in Hong Kong differ by stroke subtype. Cerebrovasc Dis. 2011;31(2):138–146. - PubMed