Partial replacement of serum with peptone and lactalbumin hydrolysate for the production of foot-and-mouth disease vaccine in BHK-21 cells

Abstract

Two batches of experimental media were prepared with Difco-Peptone and Centron-Peptone in combination with lactalbumin hydrolysate (LAH) in Eagle's salts containing 3 amino acids, vitamins and 1% bovine serum. Both medium batches supported the growth of Razi BHK-21 cells in serial passages and the replication of foot-and-mouth disease (FMD) virus type "A". The infectivity and complement fixing antigen (CFU) titres of the virus were comparable with those in Eagle's medium. The protection indices "C" of the experimental batches of vaccine were highly satisfactory. The experimental media saved up to 90% of serum in comparison with Eagle's medium.