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. 1989:(21):11-2.

dNTP imbalance and DNA double strand breaks in mouse FM3A cells and the mechanism of cell death

Y Wataya  1 , K Watanabe, S Yoshida, A Hiramoto-Yoshioka
Affiliations
  • PMID: 2575244

dNTP imbalance and DNA double strand breaks in mouse FM3A cells and the mechanism of cell death

Y Wataya et al. Nucleic Acids Symp Ser. 1989.

Abstract

The mechanism of intracellular deoxyribonucleoside-triphosphate (dNTP) imbalance death of mouse mammary tumor FM3A cells was studied. When the cells were exposed to 5-fluorodeoxyuridine, deoxyadenosine, or 2-chlorodeoxyadenosine, dNTP pool imbalance resulted. The imbalance was followed by DNA double-strand breaks and subsequent cell death. The DNA double strand breaks were directly examined by means of orthogonal-field-alternation gel electrophoresis (OFAGE). Fragmented DNA band appeared to be approximately 100-200 kbp in size. The bases of 5'-termini in the DNA were cytosine and thymine. The imbalance induced endonuclease has been isolated by DEAE-agarose column chromatography.

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