Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jul;77(7):871-3.
doi: 10.1292/jvms.14-0640. Epub 2015 Mar 8.

Detection of Escherichia albertii from chicken meat and giblets

Eriko Maeda  1 , Koichi Murakami, Nobuyuki Sera, Kenitiro Ito, Shuji Fujimoto
Affiliations

Detection of Escherichia albertii from chicken meat and giblets

Eriko Maeda et al. J Vet Med Sci. 2015 Jul.

Abstract

Escherichia albertii occasionally causes food-borne outbreaks of gastroenteritis in humans; however, little is known about the vehicle of transmission. To screen retail chicken products for the presence of E. albertii, 104 retail chicken products were investigated. Portions of enrichment cultures that were PCR-positive for E. albertii (n=3) were sub-cultured on agar medium. Only 2 strains obtained from 2 chicken giblet samples were identified as E. albertii by multi locus sequence typing. Antimicrobial susceptibility testing showed that 1 strain was resistant to streptomycin and sulfisoxazole. Both strains harbored the virulence genes cdt and eae. This study is the first description of E. albertii isolation from retail food, suggesting that chicken products are a potential vehicle of E. albertii transmission.

PubMed Disclaimer

Figures

Fig. 1.
Fig. 1.
Phylogenetic tree showing nucleotide sequence clusters of the tested Escherichia albertii strains obtained using multi-locus sequence typing. ST383, ST413, ST95 and ST2819 were included as outgroups. Reference sequences were tested in a previous study [9]. Shigella boydii serotype 13 ATCC 12032 has been reclassified into the Escherichia albertii lineage. The scale bar indicates the number of nucleotide substitutions per site.
See this image and copyright information in PMC

References

    1. Asoshima N., Matsuda M., Shigemura K., Honda M., Yoshida H., Hiwaki H., Ogata K., Oda T.2014. Identification of Escherichia albertii as a causative agent of a food-borne outbreak occurred in 2003. Jpn. J. Infect. Dis. 67: 139–140. doi: 10.7883/yoken.67.139 - DOI - PubMed
    1. Clinical and Laboratory Standards Institute. 2012. Performance standards for antimicrobial disk and dilution susceptibility tests; approved standard, tenth edition. M02-A11, CLSI, Wayne, PA.
    1. Clinical and Laboratory Standards Institute. 2014. Performance standards for antimicrobial susceptibility testing; twenty-first informational supplement. M100-S24, CLSI, Wayne, PA.
    1. Hinenoya A., Shima K., Asakura M., Nishimura K., Tsukamoto T., Ooka T., Hayashi T., Ramamurthy T., Faruque S. M., Yamasaki S.2014. Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan. BMC Microbiol. 14: 97. doi: 10.1186/1471-2180-14-97 - DOI - PMC - PubMed
    1. Huys G., Cnockaert M., Janda J. M., Swings J.2003. Escherichia albertii sp. nov., a diarrhoeagenic species isolated from stool specimens of Bangladeshi children. Int. J. Syst. Evol. Microbiol. 53: 807–810. doi: 10.1099/ijs.0.02475-0 - DOI - PubMed

Publication types

Substances