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. 2015:2015:965073.
doi: 10.1155/2015/965073. Epub 2015 Feb 8.

Identification and DUS Testing of Rice Varieties through Microsatellite Markers

Ehsan Pourabed  1 , Mohammad Reza Jazayeri Noushabadi  2 , Seyed Hossein Jamali  2 , Naser Moheb Alipour  1 , Abbas Zareyan  2 , Leila Sadeghi  2
Affiliations

Identification and DUS Testing of Rice Varieties through Microsatellite Markers

Ehsan Pourabed et al. Int J Plant Genomics. 2015.

Abstract

Identification and registration of new rice varieties are very important to be free from environmental effects and using molecular markers that are more reliable. The objectives of this study were, first, the identification and distinction of 40 rice varieties consisting of local varieties of Iran, improved varieties, and IRRI varieties using PIC, and discriminating power, second, cluster analysis based on Dice similarity coefficient and UPGMA algorithm, and, third, determining the ability of microsatellite markers to separate varieties utilizing the best combination of markers. For this research, 12 microsatellite markers were used. In total, 83 polymorphic alleles (6.91 alleles per locus) were found. In addition, the variation of PIC was calculated from 0.52 to 0.9. The results of cluster analysis showed the complete discrimination of varieties from each other except for IR58025A and IR58025B. Moreover, cluster analysis could detect the most of the improved varieties from local varieties. Based on the best combination of markers analysis, five pair primers together have shown the same results of all markers for detection among all varieties. Considering the results of this research, we can propose that microsatellite markers can be used as a complementary tool for morphological characteristics in DUS tests.

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Figures

Figure 1
Figure 1
Detecting the impurities in CMS line from its cognate isogenic maintainer line. Polymorphism between CMS (IR58025A) and maintainer line (IR58025B) of rice at mitochondrial drrcms marker.
Figure 2
Figure 2
Dendrogram of 40 rice varieties based on UPGMA cluster analysis of Dice similarity matrix calculated from 12 microsatellite markers.
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References

    1. Lombard V., Baril C. P., Dubreuil P., Blouet F., Zhang D. Genetic relationships and fingerprinting of rapeseed cultivars by AFLP: consequences for varietal registration. Crop Science. 2000;40(5):1417–1425. doi: 10.2135/cropsci2000.4051417x. - DOI
    1. Tommasini L., Batley J., Arnold G. M., et al. The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties. Theoretical and Applied Genetics. 2003;106(6):1091–1101. - PubMed
    1. Kwon Y.-S., Lee J.-M., Yi G.-B., et al. Use of SSR markers to complement tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Molecules and Cells. 2005;19(3):428–435. - PubMed
    1. Mailer R. J., Scarth R., Fristensky B. Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphisms amplified from arbitrary primers. Theoretical and Applied Genetics. 1994;87(6):697–704. doi: 10.1007/bf00222895. - DOI - PubMed
    1. de Riek J., Calsyn E., Everaert I., van Bockstaele E., de Loose M. AFLP based alternatives for the assessment of distinctness, uniformity and stability of sugar beet varieties. Theoretical and Applied Genetics. 2001;103(8):1254–1265. doi: 10.1007/s001220100710. - DOI