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. 2015:2015:635748.
doi: 10.1155/2015/635748. Epub 2015 Feb 1.

MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion

Guntulu Ak  1 , Sandra C Tomaszek  2 , Farhad Kosari  3 , Muzaffer Metintas  1 , James R Jett  4 , Selma Metintas  5 , Huseyin Yildirim  1 , Emine Dundar  6 , Jie Dong  2 , Marie Christine Aubry  7 , Dennis A Wigle  2 , Charles F Thomas Jr  8
Affiliations

MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion

Guntulu Ak et al. Biomed Res Int. 2015.

Abstract

Introduction: We investigated the expression of microRNAs and mRNAs in pleural tissues from patients with either malignant pleural mesothelioma or benign asbestos-related pleural effusion.

Methods: Fresh frozen tissues from a total of 18 malignant pleural mesothelioma and 6 benign asbestos-related pleural effusion patients were studied. Expression profiling of mRNA and microRNA was performed using standard protocols.

Results: We discovered significant upregulation of multiple microRNAs in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Hsa-miR-484, hsa-miR-320, hsa-let-7a, and hsa-miR-125a-5p were able to discriminate malignant from benign disease. Dynamically regulated mRNAs were also identified. MET was the most highly overexpressed gene in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Integrated analyses examining microRNA-mRNA interactions suggested multiple altered targets within the Notch signaling pathway.

Conclusions: Specific microRNAs and mRNAs may have diagnostic utility in differentiating patients with malignant pleural mesothelioma from benign asbestos-related pleural effusion. These studies may be particularly helpful in patients who reside in a region with a high incidence of mesothelioma.

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Figures

Figure 1
Figure 1
Representative overexpressed microRNAs.
Figure 2
Figure 2
Representative overexpressed mRNAs.
Figure 3
Figure 3
Selected pathways and genes by an integrated analysis using overexpressed mRNA (microarray) and targets genes of underexpressed miR. Yellow depicts targets of underexpressed miR and blue depicts overexpressed genes by microarray. Green depicts overexpressed genes that are also targets of underexpressed miR. There were 232 genes in the 8 pathways. Only 32 genes that participated in more than 1 pathway are shown. Only one of two pathways related to NOTCH signaling was included in creating this figure. MAPK1, TGFBR2, EP300, CDC42, MET, IGF1R, and SMAD2 participate in 3 or more pathways. MET and CCNE2 mRNAs are overexpressed and are targets of underexpressed miR.
See this image and copyright information in PMC

References

    1. Ray M., Kindler H. L. Malignant pleural mesothelioma: an update on biomarkers and treatment. Chest. 2009;136(3):888–896. doi: 10.1378/chest.08-2665. - DOI - PubMed
    1. Robinson B. W. S., Musk A. W. Benign asbestos pleural effusion: diagnosis and course. Thorax. 1981;36(12):896–900. doi: 10.1136/thx.36.12.896. - DOI - PMC - PubMed
    1. Calin G. A., Sevignani C., Dumitru C. D., et al. Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(9):2999–3004. doi: 10.1073/pnas.0307323101. - DOI - PMC - PubMed
    1. Wang D., Qiu C., Zhang H., Wang J., Cui Q., Yin Y. Human MicroRNA oncogenes and tumor suppressors show significantly different biological patterns: from functions to targets. PLoS ONE. 2010;5(9) doi: 10.1371/journal.pone.0013067.e13067 - DOI - PMC - PubMed
    1. Volinia S., Calin G. A., Liu C. G., et al. A microRNA expression signature of human solid tumors defines cancer gene targets. Proceedings of the National Academy of Sciences of the United States of America. 2006;103(7):2257–2261. - PMC - PubMed

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