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. 2015 Jan;32(1):101-6.
doi: 10.5152/balkanmedj.2015.15183. Epub 2015 Jan 1.

Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis

Tamer Sağıroğlu  1 , Mustafa Burak Sayhan  2 , Mehmet A Yağcı  3 , Tülin Yalta  4 , Gönül Sağıroğlu  4 , Elif Çopuroğlu  5 , Serhat Oğuz  1
Affiliations

Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis

Tamer Sağıroğlu et al. Balkan Med J. 2015 Jan.

Abstract

Background: Continuous ambulatory peritoneal dialysis is a successful treatment modality for patients with end-stage renal disease. Peritoneal fibrosis (PF) is the most critical complication of long-term peritoneal dialysis (PD).

Aims: In our study, we aimed to compare the effects of colchicine and sirolimus on PF induced by hypertonic peritoneal dialysis solutions in rats.

Study design: Animal experiment.

Methods: Twenty-four rats were randomly divided into three groups. The control group received an intraperitoneal injection (ip) of saline. The sirolimus group received the PD solution, plus 1.0 mg/kg/day Rapamune®. The colchicine group received the PD solution ip plus 1.0 mg/kg/day of colchicine. Blood samples were taken to measure the serum levels of VEGF, TGF-β, and TNF-α. Peritoneal tissue samples were taken for histopathological evaluation.

Results: TGF-β and TNF-α values in the sirolimus group were found to be statistically significantly lower than in the control and colchicine groups, but the differences between the control and colchicine groups were not statistically significant. No statistically significant differences were found between the groups regarding the VEGF values. Vascular neogenesis and peritoneal thickness were compared; the values in the sirolimus group were statistically reduced compared to the values in the control group. Mild fibrosis developed in 75% of all animals in the sirolimus group; there was no moderate or severe fibrosis observed. Fibrosis developed to varying degrees in 100% of the animals in the control and colchicine groups.

Conclusion: The present study demonstrates that sirolimus might be beneficial for preventing or delaying the progression of PF and neoangiogenesis. These alterations in the peritoneal membrane may be connected with reduced TNF-α and TGF-β levels.

Keywords: Colchicine; peritoneal dialysis; peritoneal fibrosis; sirolimus.

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Figures

FIG. 1. a–c.
FIG. 1. a–c.
Serum TGF-β (a), TNF-α (b), VEGF (c) measurement values in experimental groups.
FIG. 2. a, b.
FIG. 2. a, b.
The number of vessels in control group (a) and sirolimus group (b) in the sections stained immunohistochemically with CD 31
FIG. 3. a, b.
FIG. 3. a, b.
Peritoneal membrane thickness measured in control group (a) and sirolimus group (b) with hematoxylin-eosin dye
See this image and copyright information in PMC

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