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. 2012 Mar;15(1):7-11.
doi: 10.3831/KPI.2012.15.1.007.

Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells

Eun-A Choi  1 , Jeung-Won Yoon  1 , Hak-Joo Choi  2 , Dong-Hee Kim  2 , Hwa-Seung Yoo  1
Affiliations

Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells

Eun-A Choi et al. J Pharmacopuncture. 2012 Mar.

Abstract

Aim: Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.

Methods: RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs).

Results: IS4 concentration inhibited the production of interleukin-1beta (IL-1 β), interleukin-6 (IL-6), and granulocyte -macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50㎍/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF- α) stimulated by LPS.

Conclusion: IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.

Keywords: GM-CSF; IL-1β; IL-6; TNF–α; cytokine; lipopolysaccharide.

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Figures

Fig. 1
Fig. 1. Inhibitory effect of IS4 on IL-1β production in RAW 264.7 cells. Cells were incubated with LPS (1 ㎍/ml) for 24 hr. Data are expressed as percentages of the control values. Values shown are means ± SD of three independent experiments, each run in triplicate.
Fig. 2
Fig. 2. Inhibitory effect of IS4 on IL-6 production in RAW 264.7 cells. Cells were incubated with LPS (1 ㎍/ml) for 24 hr. Data are expressed as percentages of the control values. Values shown are means ± SD of three independent experiments, each run in triplicate.
Fig. 3
Fig. 3. Inhibitory effect of IS4 on TNF-α production in RAW 264.7 cells. Cells were incubated with LPS (1 ㎍/ml) for 24 hr. Data are expressed as percentages of the control values. Values shown are means ± SD of three independent experiments, each run in triplicate.
Fig. 4
Fig. 4. Inhibitory effect of IS4 on GM-CSF production in RAW 264.7 cells. Cells we-re incubated with LPS (1 ㎍/ml) for 24hr. Data are expressed as percentag-es of the control values. Values shown are means ± SD of three independe-nt experiments, each run in triplicate.
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