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. 2012 Mar;15(1):29-33.
doi: 10.3831/KPI.2012.15.1.029.

Modulatory Effects of Chrysanyhemi Flos Pharmacopuncture on Nitric-oxide (NO) Production in Murin Macrophagy Cells

Hwa-Young Shin  1 , Hyun-Jong Lee  1 , Yun-Kyu Lee  1 , Seong-Chul Lim  1 , Jae-Soo Kim  1
Affiliations

Modulatory Effects of Chrysanyhemi Flos Pharmacopuncture on Nitric-oxide (NO) Production in Murin Macrophagy Cells

Hwa-Young Shin et al. J Pharmacopuncture. 2012 Mar.

Abstract

Objectives: Much evidence exists that herbs have effective immunomodulatory activities. Chrysanthemi Flos (CF) is effective in clearing heat, reducing inflammation, dropping blood pressure and treating headache and is used as a pharmaceutical raw material for an immune enhancer. The purpose of this study was to investigate the modulatory effect of Chrysanthemi Flos pharmacopuncture on nitric-oxide (NO) production in activating macrophages.

Methods: After a murine macrophage cell line, RAW 264.7, was cultured in the presence of lipopolysaccharide (LPS), immune-modulating abilities of CF were evaluated by using NO, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) production and phagocytic activity of macrophages.

Results: CF enhanced the activities of macrophages by increasing the phagocytic activity and decreasing NO production. Especially, both LPS and CF, 200 ㎍/ml, treatment could significantly reduce the NO production, but did not change the production of IL-6 and TNF-α.

Conclusion: The results of this study indicate that CF may be of immunomodulatory value, especially for adverse diseases due to increased NO production. It may have potential for use as immunoenhancing pharmacopuncture.

Keywords: CF; RAW 264.7; herb; immunomodulatory; macrophage; nitric-oxide(NO).

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Figures

Fig. 1
Fig. 1. Cell viability of CF on RAW 264.7 cells.
Fig. 2
Fig. 2. Phagocytic activity of CF on RAW 264.7 cells.
Fig. 3
Fig. 3. NO production of CF on RAW 264.7 cells.
Fig. 4
Fig. 4. IL-6 production of CF on RAW 264.7 cells.
Fig. 5
Fig. 5. TNF-α production of CF on RAW 264.7 cells.
See this image and copyright information in PMC

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