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. 2013 Mar;16(1):30-6.
doi: 10.3831/KPI.2013.16.002.

Identification and Analysis of the Novel pGAPDH-w Gene Differentially Expressed in Wild Ginseng

Han Young-Ju  1 , Kwon Ki-Rok  2 , Kang Won-Mo  3 , Jeon Eun-Yi  3 , Jang Jun-Hyeog  3
Affiliations

Identification and Analysis of the Novel pGAPDH-w Gene Differentially Expressed in Wild Ginseng

Han Young-Ju et al. J Pharmacopuncture. 2013 Mar.

Abstract

Objective: Panax ginseng is one of the most medicinally used herbal medicines in the world. Wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the differences between wild ginseng and cultivated ginseng.

Method: To identify wild ginseng-specific genes, we used suppressive subtraction hybridization.

Results: We report that one of the clones isolated in this screen was the GAPDH(glyceraldehyde 3-phosphate dehydrogenase) gene (designated pGAPDH-w). DNA BLAST sequence analysis revealed that this pGAPDH-w gene contained novel sequences of 94 bp. RT-PCR results showed that the expression of the pGAPDH-w gene was significantly up-regulated in the wild ginseng as compared with the cultivated ginseng.

Conclusion: The pGAPDH-w gene may be one of the important markers of wild ginseng.

Keywords: PCR; cultivated ginseng; hybridization (SSH); pGAPDHw gene; suppressive subtraction; wild ginseng.

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Figures

Fig. 1
Fig. 1. Ganghwa cultivated ginseng (A), Guemsan cultivated ginseng (B) and wild mountain ginsengs used for RNA isolation (C-E).
Fig. 2
Fig. 2. Determined DNA sequence of the wild ginsengpGAPDH-w gene. Novel sequences are in red.
Fig. 3
Fig. 3. DNA sequence alignment of The pGAPDH-w gene with knownpGAPDH genes across the species. Novel sequences are in red.
Fig. 4
Fig. 4. Quantitative real time-PCR analysis of pGAPDH-w transcripts.
Fig. 5
Fig. 5. RT-PCR analysis of differential expressions of The pGAPDH-w genes.
See this image and copyright information in PMC

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